A quality-by-design approach to develop abemaciclib solid lipid nanoparticles for targeting breast cancer cell lines
Bonnie Chin1, Wei Meng Lim2, Samah Hamed Almurisi3,4
1School of Postgraduate Studies, International Medical University, Kuala Lumpur, Malaysia.
Therapeutic Delivery
|January 29, 2025
Summary
Abemaciclib-loaded solid lipid nanoparticles (ABE-SLNs) were developed using Quality-by-Design. These nanoparticles enhance anticancer drug solubility, cellular uptake, and cytotoxicity, offering a promising new delivery system.
Area of Science:
- Nanotechnology
- Pharmaceutical Sciences
- Oncology
Background:
- Abemaciclib (ABE) faces challenges with low bioavailability and multidrug resistance.
- Effective delivery systems are crucial for improving anticancer drug efficacy.
Purpose of the Study:
- To develop Abemaciclib-loaded solid lipid nanoparticles (ABE-SLNs).
- To enhance drug solubility, cellular uptake, and cytotoxicity of Abemaciclib.
- To optimize ABE-SLNs using Quality-by-Design (QbD) principles.
Main Methods:
- ABE-SLNs were prepared using melt emulsification and ultrasonication.
- Quality-by-Design (QbD) was employed for formulation optimization.
- Particle characterization included size, PDI, and zeta potential analysis.
Main Results:
- Optimized ABE-SLNs (Precirol-ATO5, Brij-58) showed a particle size of 170.4 nm, PDI of 0.25, and zeta potential of -26.4 mV.
- ABE-SLNs demonstrated sustained drug release and high entrapment efficiency (79.96%).
- Enhanced anticancer activity was observed in MDA-MB-231 and T47D cells; increased cellular uptake in Caco-2 cells.
Conclusions:
- Quality-by-Design enabled high entrapment efficiency and sustained release of ABE-SLNs.
- ABE-SLNs significantly improved cellular uptake and cytotoxicity compared to free Abemaciclib.
- This nanoparticle approach offers a novel and potentially superior method for cancer drug delivery.
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