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Updated: May 30, 2025
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Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling
Published on: August 3, 2018
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Transforming growth factor beta induces interleukin-11 expression in osteoarthritis
Juliane Lokau1, Miriam Bollmann2, Yvonne Garbers3
1Institute of Clinical Biochemistry, Hannover Medical School, 30625 Hannover, Germany; Department of Pathology, Otto-von-Guericke-University Magdeburg, 39120 Magdeburg, Germany.
Cytokine
|January 29, 2025
Summary
Interleukin-11 (IL-11) is elevated in osteoarthritis (OA) synovial fluid, produced by chondrocytes and fibroblasts, and activates these cells via IL-11R, suggesting IL-11 as a potential OA therapeutic target.
Area of Science:
- Immunology
- Cell Biology
- Rheumatology
Background:
- Interleukin-11 (IL-11) is a cytokine with complex roles in inflammation.
- Its expression patterns and targets in inflammatory diseases like osteoarthritis (OA) are not well understood.
- IL-11 signaling involves IL-11R and gp130, determining cellular responsiveness.
Purpose of the Study:
- To investigate the role of IL-11 in osteoarthritis.
- To identify cells expressing and responding to IL-11 in OA.
- To explore IL-11 as a potential therapeutic target for OA.
Main Methods:
- Enzyme-linked immunosorbent assay (ELISA) to quantify IL-11 in synovial fluid.
- Immunohistochemistry to detect IL-11 and IL-11R expression in OA tissues.
- Primary cell culture of chondrocytes and fibroblasts for stimulation assays.
- Analysis of Jak/STAT3 signaling pathway activation.
Main Results:
- Elevated IL-11 levels were found in OA synovial fluid compared to rheumatoid arthritis (RA).
- IL-11 is expressed by chondrocytes in OA cartilage, induced by TGF-β1.
- Both cartilage and synovium express IL-11R, and OA chondrocytes/fibroblasts activate Jak/STAT3 in response to IL-11.
Conclusions:
- IL-11 is significantly present and active in osteoarthritis.
- Chondrocytes and fibroblasts are both sources and targets of IL-11 in OA.
- IL-11 represents a promising new therapeutic target for osteoarthritis treatment.
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