Detection of biogenic amines using a ylidenemalononitrile enamine-based fluorescence probe: Applications in food...

Biogenic amines (BAs) are a class of nitrogen-containing natural organic compounds. Elevated levels of BAs are a reliable indicator of food spoilage and pose a significant risk to human health. Thus, the development of real-time sensors for BAs monitoring is crucial. In this study, we present a novel fluorescence probe, TP-A, based on ylidenemalononitrile enamine, which can detect various BAs including putrescine, cadaverine, and spermine. TP-A exhibits a significant fluorescence intensity increase at 600 nm in the presence of these BAs in an aqueous system, which can effectively monitor BAs in representative vegetable onion tissues. Notably, TP-A-loaded filter paper has been developed into test strips for real-time monitoring of BAs released from shrimp under different temperatures. This work offers a precise method for probing the release of BAs in real food samples, which is promising for application in food quality control.