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Published on: May 15, 2019
Sustainable utilization of bovine adipose tissue derivatives as robust antimicrobial agents against
Muhammed Abdelhameed Ismael Alcici1, Salma Waheed Abdelhaleem2, Karima Mogahed Fahim3
1Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Background:
The excessive use of antibiotics is a major contributor to the global issue of antimicrobial resistance (AMR), a significant threat to human and animal health. Hence, assessing new strategies for managing Multi-Drug Resistant (MDR) microorganisms is vital. In this study, the use of mechanically isolated mature adipose cells (MIMACs) and their lysate (Adipolysate) as a new sustainable antimicrobial agent was assessed against Methicillin-resistant Staphylococcus aureus (MRSA).
Conclusions:
The minimum volume of MIMACs achieved complete bacterial inhibition (Minimum Lethal volume) was 75 µl and 100 µl for bacterial concentration of 1010 and 1012 cfu/ml, respectively. Direct bacterial membrane attachment and intracellular capture was visualized under light and electron microscopy. Adipolysate was characterized via GC-MS, the fatty acid profile demonstrated several components with known antimicrobial properties. The tested Adipolysate revealed inhibition zone of diameter 25.33 ± 0.88 mm against the tested S. aureus strain, compared with the inhibition zone of Vancomycin (24.0 ± 0.00 mm) and Erythromycin (30.0 ± 0.00). The study revealed the potential effects of MIMACs and Adipolysate as sustainable, natural, and robust antimicrobial agents. However, these preliminary results will be further investigated to understand the mechanism of action and explore possible applications in various fields.
Insights
Mechanically isolated mature adipose cells (MIMACs) and Adipolysate show potential as natural antimicrobial agents against Methicillin-resistant Staphylococcus aureus (MRSA). These agents demonstrate bacterial membrane attachment and inhibition comparable to conventional antibiotics.
Area of Science:
- Biomedical Science
- Microbiology
- Biotechnology
Background:
- Antimicrobial resistance (AMR) is a global health threat driven by excessive antibiotic use.
- Developing new strategies to combat Multi-Drug Resistant (MDR) microorganisms is crucial.
- Mechanically isolated mature adipose cells (MIMACs) and their lysate (Adipolysate) were investigated as novel antimicrobial agents.
Purpose of the Study:
- To evaluate the antimicrobial efficacy of MIMACs and Adipolysate against Methicillin-resistant Staphylococcus aureus (MRSA).
- To characterize the antimicrobial properties of Adipolysate.
- To explore the potential of adipose-derived materials as sustainable antimicrobial solutions.
Main Methods:
- Assessment of MIMACs' Minimum Lethal volume against MRSA at different bacterial concentrations.
- Microscopic visualization (light and electron) of bacterial-cell interactions.
- Gas Chromatography-Mass Spectrometry (GC-MS) analysis of Adipolysate's fatty acid profile.
- Determination of inhibition zones for Adipolysate, Vancomycin, and Erythromycin against S. aureus.
Main Results:
- Specific volumes of MIMACs (75 µl and 100 µl) achieved complete MRSA inhibition at 10^10 and 10^12 cfu/ml, respectively.
- Direct attachment and intracellular capture of bacteria by MIMACs were observed.
- Adipolysate's fatty acid profile revealed components with known antimicrobial activity.
- Adipolysate exhibited an inhibition zone comparable to Vancomycin and Erythromycin.
Conclusions:
- MIMACs and Adipolysate demonstrate significant potential as sustainable and natural antimicrobial agents.
- The findings suggest a direct interaction mechanism involving bacterial membrane attachment.
- Further research is warranted to elucidate the precise mechanism of action and explore diverse applications.
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