Exploring the role of oxidative stress in carotid atherosclerosis: insights from transcriptomic data and single-cell sequencing combined with machine learning

Yiqin Yang ¹, Mei Dong ^2,3

⁰The Second School of Clinical Medicine, Shandong University, Jinan, China.

Biology Direct +

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January 29, 2025

View abstract on PubMed

Summary

This study identifies key oxidative stress genes (IDH1, CD36) and immune cells in carotid atherosclerotic plaque formation. These findings offer insights into atherosclerosis mechanisms and potential therapeutic targets.

Area Of Science

Biomedical research
Bioinformatics
Immunology

Background

Carotid atherosclerotic plaque is a major cause of cardiovascular and cerebrovascular diseases.
Plaque development is linked to oxidative stress and immune inflammation.
Understanding these factors is crucial for disease management.

Purpose Of The Study

To identify key oxidative stress-related genes in carotid plaque.
To pinpoint critical immune cell infiltrations in plaque development.
To explore the interplay between oxidative stress and immune cells in atherosclerosis.

Main Methods

Bioinformatic analysis of gene expression data.
Single-cell data analysis to identify immune cell types.
Integration of predictive modeling with molecular data.

Main Results

Up-regulation of oxidative stress genes (e.g., IDH1, CD36) was observed.
These genes play a significant role in resident-like and foam macrophages.
This highlights their importance in plaque formation and progression.

Conclusions

Oxidative stress and immune inflammation are key drivers of plaque formation, progression, and stabilization.
The study provides novel insights into carotid atherosclerosis mechanisms.
Identified genes and pathways may serve as potential therapeutic targets for atherosclerosis.

Keywords:

Immune inflammation Machine learning Oxidative stress Single-cell sequencing