Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

16.5K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.5K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

9.7K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
9.7K
Experimental RNAi02:15

Experimental RNAi

6.0K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.0K
Types of RNA01:23

Types of RNA

63.1K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.1K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

6.3K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
6.3K
Leaky Scanning02:28

Leaky Scanning

5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integration of multi-omics for precision therapy in breast cancer.

Drug discovery today·2026
Same author

Navigating luminal heterogeneity: etiology-based proteogenomic subtyping for targeted treatment strategies in breast cancer.

Molecular cancer·2026
Same author

CDK1-mediated phosphorylation of EPLIN is crucial for mitotic spindle positioning.

Communications biology·2026
Same author

Comprehensive Evaluation of the Anti-Inflammatory Activity of Ling-Zhi-8 (LZ-8) In Vitro and a Rheumatoid Arthritis Animal Model.

Food science & nutrition·2026
Same author

Microwave-Accelerated Proteolysis Minimizes Artificial Deamidation and Enhances Speed for Peptide Mapping of Protein Pharmaceuticals.

Rapid communications in mass spectrometry : RCM·2026
Same author

Comparative Metabolomic Profiling of Roasted Coffee Beans From Taiwan and Brazil.

Rapid communications in mass spectrometry : RCM·2026
Same journal

Variability and population structure of watermelon mosaic virus in zucchini crops in Poland.

The Journal of general virology·2026
Same journal

Probing the sequence constraints for the stable incorporation of chimeric NSP1 segments into infectious rotaviruses.

The Journal of general virology·2026
Same journal

Assessment of influenza virus and coronavirus tropism, replication competence and disease severity in <i>ex vivo</i> and <i>in vitro</i> cultures of the human respiratory tract.

The Journal of general virology·2026
Same journal

GRHAL1, a novel lncRNA, regulates HIV-1 gene expression by modulating Tat- and Sp1-mediated HIV-1 LTR activation.

The Journal of general virology·2026
Same journal

Hepatitis B virus X protein induces E6-associated protein-mediated proteasomal degradation of p53 phosphorylated at Ser-15.

The Journal of general virology·2026
Same journal

Sensing the storm: how inflammatory signalling drives reactivation of the human cytomegalovirus major immediate-early promoter.

The Journal of general virology·2026
See all related articles

Related Experiment Video

Updated: May 30, 2025

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
05:23

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

6.0K

Linking the function of cis-acting RNA elements to coronavirus replication using interactomes.

Yueh-Chun Yao1, Chun-Chun Yang1, Meilin Wang2,3

  • 1Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University, Taichung 40227, Taiwan, ROC.

The Journal of General Virology
|January 30, 2025
PubMed
Summary
This summary is machine-generated.

This study reveals that cis-acting RNA elements in coronaviruses are crucial for replication. Their interactome architecture and associated biological processes directly correlate with viral replication efficiency.

Keywords:
cis-acting RNA elementscoronavirusinteractomepositive-strand RNA virusreplication

More Related Videos

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
10:53

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

9.0K
In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
10:27

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

1.5K

Related Experiment Videos

Last Updated: May 30, 2025

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
05:23

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

6.0K
Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
10:53

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

9.0K
In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
10:27

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

1.5K

Area of Science:

  • Molecular Biology
  • Virology
  • Genomics

Background:

  • Cis-acting RNA elements in the 3' UTR of coronaviruses are vital for replication.
  • The precise mechanism linking these RNA elements to replication is not fully understood.

Purpose of the Study:

  • To investigate the relationship between cis-acting RNA elements, their interactomes, and coronavirus replication.
  • To establish a model for analyzing cis-acting RNA elements in RNA virus replication.

Main Methods:

  • Comparative analysis of coronavirus 3' UTR cis-acting RNA elements, their interactomes, and replication efficiency.
  • Utilized the compound 5-benzyloxygramine to alter the interactome and assess its impact on replication.

Main Results:

  • Biological processes like translation, protein folding, and stabilization are linked to the cis-acting RNA element interactome.
  • The architecture of cis-acting RNA elements and their interactomes strongly correlates with coronavirus replication efficiency.
  • Altering the interactome with 5-benzyloxygramine significantly reduced coronavirus replication.

Conclusions:

  • Cis-acting RNA elements are directly linked to coronavirus replication through their interactomes.
  • A novel model is proposed for analyzing cis-acting RNA elements in RNA virus replication using interactome data.