Technologies for studying phase-separated biomolecular condensates

Biomolecular condensates, also referred to as membrane-less organelles, function as fundamental organizational units within cells. These structures primarily form through liquid-liquid phase separation, a process in which proteins and nucleic acids segregate from the surrounding milieu to assemble into micron-scale structures. By concentrating functionally related proteins and nucleic acids, these biomolecular condensates regulate a myriad of essential cellular processes. To study these significant and intricate organelles, a range of technologies have been either adapted or developed. In this review, we provide an overview of the most utilized technologies in this rapidly evolving field. These include methods used to identify new condensates, explore their components, investigate their properties and spatiotemporal regulation, and understand the organizational principles governing these condensates. We also discuss potential challenges and review current advancements in applying the principles of biomolecular condensates to the development of new technologies, such as those in synthetic biology.