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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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Related Experiment Video

Updated: May 30, 2025

Author Spotlight: Exploring Cellular Zinc Regulation Through ZnT1 Functionality
07:55

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A highly sensitive and colorimetric fluorescent sensor for visualizing Zn2+ in aqueous solution.

Zixuan Yuan1, Yu Zhang1, Wenjing Qu1

  • 1College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006 China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|January 30, 2025
PubMed
Summary

A novel sensor, QPEBT, detects zinc ions (Zn2+) with high sensitivity and visual color changes. This fluorescent sensor is effective for real-world applications, including water quality monitoring and biological imaging.

Keywords:
Colorimetric detectionFluorescence enhancementQuinolimideVisualizationZn(2+) sensor

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Area of Science:

  • Chemical Sensors
  • Fluorescent Probes
  • Materials Science

Background:

  • Zinc ions (Zn2+) play crucial roles in biological systems.
  • Developing selective and sensitive sensors for Zn2+ is vital for research and diagnostics.
  • Existing methods for Zn2+ detection can be limited by sensitivity, selectivity, or complexity.

Purpose of the Study:

  • To develop a new colorimetric fluorescent sensor, QPEBT, for sensitive and selective detection of Zn2+.
  • To investigate the sensing mechanism and performance of QPEBT.
  • To evaluate the practical applicability of QPEBT in environmental and biological samples.

Main Methods:

  • Synthesis of the QPEBT sensor.
  • Spectroscopic studies (fluorescence, UV-Vis) for sensing mechanism.
  • Stoichiometry determination (Job's plot, HRMS).
  • Limit of detection (LOD) and pH range analysis.
  • Application testing in aqueous solutions, test strips, and biological samples.

Main Results:

  • QPEBT exhibited sensitive fluorescence enhancement and distinct color changes upon binding with Zn2+.
  • The sensor demonstrated a 1:1 binding stoichiometry with Zn2+.
  • QPEBT showed a low LOD (47.4 nM) and effective detection within a pH range of 6.4-9.6.
  • Visual detection and quantitative analysis of Zn2+ were achieved using naked-eye observation and RGB analysis.
  • Successful application in monitoring Zn2+ in water samples and fluorescence imaging in cells and sprouts.

Conclusions:

  • QPEBT is a highly effective colorimetric fluorescent sensor for Zn2+ detection.
  • The sensor offers rapid, reversible, and sensitive detection with practical applicability.
  • QPEBT holds promise for real-time monitoring of Zn2+ in diverse environmental and biological settings.