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CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation
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Exosomes-mediated CRISPR/Cas delivery: A cutting-edge frontier in cancer gene therapy

Bhavanisha Rithiga S1, Rajib Dhar1, Arikketh Devi1

  • 1Cancer and Stem Cell Biology Laboratory, Department of Genetic Engineering, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu District, TamilNadu 603203, India.

Gene
|January 30, 2025
PubMed

Insights

CRISPR/Cas gene editing shows promise for cancer therapy but faces challenges. Engineering CRISPR into exosomes enhances specificity and reduces side effects, improving its potential for treating cancer.

Area of Science:

  • Biotechnology
  • Genetics
  • Oncology

Background:

  • Cancer is a leading global disease with ongoing research into novel treatments.
  • CRISPR/Cas gene editing offers targeted therapy potential but struggles with specificity and delivery.
  • Extracellular vesicles (EVs), particularly exosomes, are key in intercellular communication.

Purpose of the Study:

  • To review the application of exosomes and CRISPR/Cas systems in cancer research.
  • To explore the use of exosome-mediated CRISPR/Cas delivery for cancer treatment.
  • To discuss the future prospects of this therapeutic approach.

Main Methods:

  • Review of current literature on CRISPR/Cas systems and exosomes in cancer.
  • Analysis of exosome engineering for CRISPR/Cas component encapsulation.
  • Evaluation of exosome-based CRISPR/Cas delivery systems for enhanced cancer therapy.

Main Results:

  • Exosomes improve CRISPR/Cas system specificity and reduce off-target effects.
  • Exosome delivery enhances the therapeutic efficacy of CRISPR/Cas in cancer models.
  • Engineered exosomes overcome limitations of direct CRISPR/Cas application.

Conclusions:

  • Exosome-mediated CRISPR/Cas delivery represents a promising strategy for advanced cancer treatment.
  • This approach enhances gene editing precision and therapeutic outcomes.
  • Further research is warranted to optimize exosome-based CRISPR/Cas for clinical application.
Keywords:
CRISPR/CasCancerExosomesTherapeutic potential

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