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[Development and validation of a conductimetric method for determining traces of detergent in final rinse water]
Safae Alaoui1, Ismail Bennani1, Sara Hajjaj1
1Faculté de médecine, de pharmacie et de médecine dentaire de Fès, B.P. 1893, Km 2.200, Route de Sidi Harazem, 30000 Fès, Maroc; Centre hospitalier universitaire Hassan II de Fès, BP 1835, 30000 Fès, Maroc.
Objective:
The aim of our work is to develop a simple, rapid and inexpensive method for the detection and quantification of a detergent (phosphoric acid) in final rinse water using conductivity.
Methods:
The conductimetric method was used. A calibration curve was obtained by measuring the conductivity of successive dilutions of a stock solution of the detergent. The conductivity of the sample was measured using the water from the last rinse. Detergent concentration in the sample was calculated using the regression equation of the calibration curve. Analytical validation was carried out in accordance with international harmonization conference guidelines ICH Q2. Data analysis was performed using jamovi software version 2.3.28.
Results:
Linearity was proved by the correlation between concentration and conductivity with r=0.98. The coefficient of variation for repeatability and intermediate precision were low, and the mean recovery rates ranged from 98 to 102%. Calculated sample concentrations were below 5ppm.
Conclusions:
The conductimetric method proposed in our study has been validated for the determination of phosphoric acid. It has the advantage of being rapid, inexpensive and simple. This makes the method the first choice for the determination of trace amounts of detergent in the pharmaceutical and research laboratories.
