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DM9CP-8 upon binding microbes activates MASPL-1-C3 axis to regulate the mRNA expressions of IL17s in oysters

Yinan Li1, Jiejie Sun2, Wenwen Yang1

  • 1College of Life Sciences, Liaoning Normal University, Dalian 116029, Liaoning, China; Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China; Liaoning Key Laboratory of Aquatic Disease Prevention and Control, Dalin Ocean University, Dalian 116023, China.

International Journal of Biological Macromolecules
|January 30, 2025
PubMed

Insights

The oyster DM9 domain-containing protein (DM9CP) acts as a pattern recognition molecule, binding microbes and activating immune pathways. This protein regulates inflammation-related signaling and exhibits antibacterial properties in oysters.

Area of Science:

  • Marine biology
  • Immunology
  • Molecular biology

Background:

  • DM9 domain-containing proteins (DM9CPs) are pattern recognition molecules involved in invertebrate immune responses.
  • Understanding the role of DM9CPs in shellfish immunity is crucial for aquaculture and disease management.

Purpose of the Study:

  • To identify and characterize a novel DM9CP (CgDM9CP-8) from the oyster Crassostrea gigas.
  • To investigate the function of CgDM9CP-8 in pattern recognition, microbial binding, and immune signaling.

Main Methods:

  • Identification and cloning of CgDM9CP-8 from Crassostrea gigas.
  • Recombinant protein expression and binding assays with PAMPs and microbes.
  • Gene expression analysis (mRNA) in oyster haemocytes after stimulation.
  • In vitro antibacterial assays and interaction studies with complement CgC3.
  • RNA interference (RNAi) to assess the in vivo function of CgDM9CP-8.

Main Results:

  • CgDM9CP-8 mRNA was highly expressed in haemocytes.
  • Recombinant CgDM9CP-8 bound to various PAMPs (MAN, PGN, LPS) and microbes (Vibrio splendidus, E. coli, etc.).
  • CgDM9CP-8 expression was upregulated upon LPS or V. splendidus stimulation.
  • CgDM9CP-8 inhibited microbial growth in vitro and activated complement CgC3.
  • RNAi-mediated knockdown of CgDM9CP-8 reduced interleukin 17 (IL17) expression after V. splendidus challenge.

Conclusions:

  • CgDM9CP-8 functions as a pattern recognition molecule in oysters, binding to microbial components.
  • CgDM9CP-8 possesses antibacterial activity and modulates the complement system (CgC3).
  • CgDM9CP-8 regulates the expression of interleukin 17s (CgIL17s), indicating a role in inflammatory responses.
Keywords:
Complement systemDM9 domainInterleukin 17OystersPattern recognition receptor

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