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Enhancing nanopore adaptive sampling for PromethION using readfish at scale
Rory Munro1, Alexander Payne1, Nadine Holmes2
1School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, United Kingdom.
Genome Research
|January 30, 2025
Abstract
None:
A unique feature of Oxford Nanopore Technologies sequencers, adaptive sampling, allows precise DNA molecule selection from sequencing libraries. Here, we present enhancements to our tool, readfish, enabling all features for the industrial scale PromethION sequencer, including standard and "barcode-aware" adaptive sampling. We demonstrate effective coverage enrichment and assessment of multiple human genomes for copy number and structural variation on a single PromethION flow cell.

