Identification and Profiling of Primary Metabolites Through GC-MS and Associated Data Processing

This chapter presents a comprehensive approach to profiling plant-derived primary metabolites using metabolomics, highlighting its critical role in decoding the biosynthesis of bioactive plant compounds. It details the utilization of gas chromatography-mass spectrometry (GC-MS) for the effective analysis and profiling of these metabolites. The process, encompassing extraction methods, chemical derivatization, and data processing, is thoroughly outlined. This methodology outlines comprehensive procedures for each stage of the workflow, encompassing metabolite extraction, GC-MS analysis, and data alignment, to produce a metabolomics dataset.