Analysis and Identification of Polyphenols in Fragaria, Rubus, and Ribes Species by Nontargeted UHPLC-PDA-MS...

Profiling of secondary metabolites within Fragaria sp. (strawberry), Rubus sp. (raspberries and blackberries), and Ribes sp. (blackcurrants and redcurrants) is of key importance with respect to the nutritional (antioxidant) benefits associated with a range of polyphenol classes within such fruits and how they vary between growth environment and season, as well as their deployment as quantitative trait loci (QTL) within breeding populations. This chapter presents an ultra-high-performance liquid chromatography (UHPLC) approach applying aqueous functionalized C18 chromatography combined with mass spectrometry (MS) for the characterization and relative quantification of a wide range of polyphenols such as the pelargonidins, being rich in strawberry; cyanidins, procyanidins, and anthocyanins, being rich in raspberry; and delphinidins and prodelphinidins, being particularly rich in blackcurrant, as well as a range of colorless flavonoids, and amino and organic acids.