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Published on: November 1, 2024
CCDC154 knockdown inhibits growth of liver cancer via suppressing expression of Snail
Rulan Ma1, Hongmei Nie2, Caijing Mo1
1Department of Surgical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yanta Road, Xi'an, 710061, Shaanxi, China.
Objective:
The effect of coiled-coil domain-containing 154 (CCDC154) in liver cancer (LC) remains unexplored. The objective of this study was to investigate the role of CCDC154 in LC and its underlying mechanism.
Methods:
The analysis of CCDC154 expression and prognosis was performed using UALCAN, Human Protein Atlas and Kaplan-Meier plotter websites. Protein expression was measured using Western blotting assay. Lentivirus was used to silence CCDC154 expression in LC cells. The proliferation and apoptosis of LC cells was evaluated by cell counting assay, colony formation assay and flow cytometry. The migration and invasion of LC cells were investigated using scratch wound-healing assay and Transwell assay.
Results:
The results showed that CCDC154 was highly expressed in LC and related to tumor grade and stage. High CCDC154 expression was associated with to poor outcomes in LC patients. Silencing of CCDC154 inhibited proliferation, migration and invasion of LC cells. It also increased apoptosis in LC cells. After CCDC154 knockdown, the expression of Twist, Vimentin and Snail was down-regulated. Overexpression of Snail abated the inhibitory caused by CCDC154 knockdown on LC cell growth.
Conclusion:
CCDC154 knockdown suppressed LC development through reducing Snail expression.
Insights
Coiled-coil domain-containing 154 (CCDC154) is highly expressed in liver cancer (LC), promoting tumor growth. Silencing CCDC154 inhibits LC cell proliferation, migration, and invasion by reducing Snail expression.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- The role of coiled-coil domain-containing 154 (CCDC154) in liver cancer (LC) is currently unknown.
- Investigating novel molecular targets is crucial for advancing liver cancer treatment strategies.
Purpose of the Study:
- To elucidate the function of CCDC154 in liver cancer.
- To explore the underlying molecular mechanisms by which CCDC154 influences liver cancer progression.
Main Methods:
- Bioinformatic analysis of CCDC154 expression and prognosis using UALCAN, Human Protein Atlas, and Kaplan-Meier plotter.
- Western blotting for protein expression analysis.
- Lentiviral silencing of CCDC154 in liver cancer cells.
- Functional assays including cell counting, colony formation, flow cytometry, scratch wound-healing, and Transwell assays to assess proliferation, apoptosis, migration, and invasion.
Main Results:
- CCDC154 is upregulated in liver cancer and correlates with advanced tumor grade and stage.
- High CCDC154 expression is associated with poorer patient outcomes.
- CCDC154 knockdown significantly inhibited proliferation, migration, and invasion while enhancing apoptosis in liver cancer cells.
- Knockdown of CCDC154 led to decreased expression of Twist, Vimentin, and Snail.
- Snail overexpression partially rescued the inhibitory effects of CCDC154 knockdown on cell growth.
Conclusions:
- CCDC154 knockdown suppresses liver cancer development.
- The tumor-suppressive effect of CCDC154 knockdown is mediated through the downregulation of Snail expression.
