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Updated: May 30, 2025
Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization
Published on: September 1, 2018
Participation of COX2/mPGES1/PGE2 in mouse and human endometrial stromal decidualization
Peng-Chao Wang1, Jie Liu2, Yue-Fang Liu3
1College of Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, Jinzhong, China.
Background:
Prostaglandin E2 (PGE2) is vital for embryo implantation and decidualization. Whether COX2/mPGES1/PGE2 pathway is essential for mouse and human decidualization remains unclear.
Results:
This study showed that mPGES1 was highly expressed in the mouse uterus's subluminal stromal cells at the implantation site. COX2-specific inhibitor Valdecoxib and mPGES1 selective inhibitor MK886 were used to analyze the roles of mPGES1 and COX2 during mouse and human decidualization. During mouse in vitro decidualization, decidua/trophoblast prolactin-related protein (Dtprp) expression was significantly suppressed by Valdecoxib and MK886. Under human in vitro decidualization, mPGES1 significantly increases, while both cPGES and mPGES2 remain unchanged. PGE2-mediated upregulation of insulin growth factor binding protein 1 (IGFBP1) was significantly inhibited by Valdecoxib and MK886.
Conclusions:
Our findings suggest the involvement of COX2/mPGES1/PGE2 pathway in both mouse and human decidualization.
