Related Experiment Video
Updated: May 30, 2025

Laser-free Hydroxyl Radical Protein Footprinting to Perform Higher Order Structural Analysis of Proteins
Published on: June 4, 2021
Hydroxyl Radical-π Interaction in a Single Crystal
Mohit Kulshrestha1, Abhijit Nandy2, Shibdas Banerjee2
1Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India.
Abstract:
Numerous attempts for organic radical stability mostly entail steric hindrance, spin-delocalization, supramolecular interaction with the host, π-π interactions, and hydrogen bonding. To date, there is no report of single crystals containing a hydroxyl radical (•OH). In this work, we have stabilized •OH in the crystal, which has been obtained from the filtrate after separating the precipitate of the chromenopyridine radical (DCP(2)•) from the reaction mixture. DCP(2)• abstracts a hydrogen atom from dissolved water in the ethanolic filtrate to grow the single crystal containing DCPH(2) and •OH in the asymmetric unit. The crystal packing and computational studies suggest that π-•OH and •OH···N hydrogen-bonding interactions are responsible for stabilizing •OH. The presence of •OH has been further confirmed by mass analysis with the 2,2,6,6-tetramethylpiperidin-1-yl)oxyl (TEMPO) adduct. Solid-state electron paramagnetic resonance (EPR), solution state nitroblue tetrazolium (NBT) assay, and spin trapping with 5,5-dimethyl-1-pyrroline N-oxide (DMPO) in the presence of super oxide dismutase suggest •OH formation in the single crystal.
More Related Videos
10:52Multiscale Sampling of a Heterogeneous Water/Metal Catalyst Interface using Density Functional Theory and Force-Field Molecular Dynamics
Published on: April 12, 2019
13:41Monitoring Equilibrium Changes in RNA Structure by 'Peroxidative' and 'Oxidative' Hydroxyl Radical Footprinting
Published on: October 17, 2011
Related Concept Videos
Radical Formation: Homolysis
Radical Reactivity: Overview
Radical Formation: Overview
Radicals from spin-paired molecules:
Radicals can be obtained from spin-paired molecules either by homolysis or electron transfer. While two radicals are formed in the former, an electron is added in the...
π Molecular Orbitals of the Allyl Radical
The allyl systems have identical molecular orbitals but differ in the number of π electrons....
Radical Formation: Abstraction
Even though homolysis produces radicals, it is different from radical...
Radical Reactivity: Intramolecular vs Intermolecular