Related Experiment Video

Updated: Aug 1, 2026

Clean Sampling and Analysis of River and Estuarine Waters for Trace Metal Studies
10:44

Clean Sampling and Analysis of River and Estuarine Waters for Trace Metal Studies

Published on: July 1, 2016

Simple automated wet digestion of animal tissues for determination of seven elements by atomic absorption

C D Salisbury, W Chan

    Journal - Association of Official Analytical Chemists
    |March 1, 1985
    PubMed

    More Related Videos

    Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells13:04

    Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells

    Published on: May 16, 2019

    Preparation of Food Samples Using Homogenization and Microwave-Assisted Wet Acid Digestion for Multi-Element Determination with ICP-MS06:53

    Preparation of Food Samples Using Homogenization and Microwave-Assisted Wet Acid Digestion for Multi-Element Determination with ICP-MS

    Published on: December 22, 2023

    Related Concept Videos

    Atomic Absorption Spectroscopy: Overview01:27

    Atomic Absorption Spectroscopy: Overview

    Atomic absorption spectroscopy (AAS) is a technique used to analyze elements by measuring electromagnetic radiation (EMR) absorbed by atoms, which causes them to transition to a higher-energy orbit. The most crucial step in AAS is atomization, where the analyte is converted into gas-phase atoms, typically through a flame or furnace. Some of these atoms become thermally excited in the flame, while most remain in the ground state.
    When irradiated by EMR of a particular wavelength, these...
    Atomic Absorption Spectroscopy: Atomization Methods01:25

    Atomic Absorption Spectroscopy: Atomization Methods

    Atomic Absorption Spectroscopy (AAS) atomizes samples through flame atomization or electrothermal atomization. Flame atomization typically involves a nebulizer and spray chamber assembly to combine the sample with a fuel–oxidant mixture, creating a fine aerosol mist that enters a burner. Typically, the fuel and oxidant are combined in an approximately stoichiometric ratio. However, for atoms that are easily oxidized, a fuel-rich mixture may be more advantageous. Only about 5% of the aerosol...
    Atomic Absorption Spectroscopy: Lab01:21

    Atomic Absorption Spectroscopy: Lab

    For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
     Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...
    Atomic Emission Spectroscopy: Overview01:20

    Atomic Emission Spectroscopy: Overview

    Atomic emission spectroscopy (AES) is an analytical technique used to determine the elemental composition of a sample by analyzing the light emitted from excited atoms. In AES, atoms in a sample are excited to higher energy levels by thermal energy from high-temperature sources, such as plasma, arcs, or sparks. When these excited atoms return to lower energy states, they emit light at specific wavelengths characteristic of each element. The resulting atomic emission spectrum, which consists of...
    Atomic Emission Spectroscopy: Instrumentation01:22

    Atomic Emission Spectroscopy: Instrumentation

    The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
    Atomic Emission Spectroscopy: Lab01:29

    Atomic Emission Spectroscopy: Lab

    AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...