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Updated: May 30, 2025

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Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining
Published on: June 23, 2020
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Difunctional oxidatively cleavable alkenyl boronates: application to cellular peroxide sensing from a
Brittany M Klootwyk1, Grace M Fleury1, Savannah Albright1
1Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA. florean@pitt.edu.
Abstract:
This manuscript describes the development of difunctional alkenyl boronates that contain an oxidatively releasable cargo and an amine for attaching to groups that can improve physical properties or enhance cellular targeting. The design is applied to a FRET-based system that delivers a selective fluorescence response in oxidatively stressed cells.

