Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Drug Discovery: Overview

Drug Discovery: Overview

Drug discovery is a multifaceted process involving extensive screening, testing, and optimization of lead compounds to identify potential new drugs for therapeutic use. It combines several approaches, including screening large numbers of natural products, chemical modification of known active molecules, identification of new drug targets, and rational design based on biological mechanisms and drug-receptor structure. These approaches are carried out in both academic research laboratories and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Serum levels of the C-terminal pro-peptide of type V collagen have prognostic potential and are associated with a normal subtype of desmoplasia in a retrospective cohort of patients with esophagogastric cancer.

Therapeutic advances in medical oncology·2026

Same author

Epithelial PCSK6 Promotes Proliferation and Decreases Collagen Deposition by Fibroblasts Potentially via MMP Activation.

International journal of molecular sciences·2026

Same author

Zigakibart Potently Inhibits A Proliferation-Inducing Ligand-Mediated IgA Production, Addressing IgAN Pathogenesis Root Causes.

Kidney international reports·2026

Same author

Imbalances of mitochondrial dynamics in solid tumors.

Oncogenesis·2026

Same author

Transcriptome-Based Classification of Resected Pancreatic Ductal Adenocarcinoma Enhances Prognostic Modelling Accuracy of Overall Survival Following Adjuvant Treatment.

International journal of cancer·2026

Same author

Thermally enhanced efficacy of chemotherapy potentiates cisplatin-based hyperthermic intraperitoneal chemotherapy in ovarian cancer.

Scientific reports·2026

Search research articles

Related Experiment Video

Updated: Jun 12, 2026

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

Published on: March 3, 2015

Identification of C/EBPδ-Modifying Compounds as Potential Anticancer Agents Using a High-Throughput Drug Screen.

Leonie Hartl^1,2, JanWillem Duitman^3,4,5, Hella L Aberson^1,2

¹Laboratory for Experimental Oncology and Radiobiology, Center for Experimental and Molecular Medicine, Amsterdam UMC Location University of Amsterdam, Amsterdam, The Netherlands.

Journal of Cellular and Molecular Medicine

|January 31, 2025

Summary

Researchers identified new compounds that modulate CCAAT/enhancer-binding protein delta (C/EBPδ) activity. These compounds, including cell cycle inhibitors, show potential for targeting pancreatic ductal adenocarcinoma (PDAC) progression.

Keywords:

Akt CCAAT/enhancer‐binding protein delta PI3K cyclin‐dependent kinase drug screen mTOR pancreatic ductal adenocarcinoma

More Related Videos

A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput

A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput

Published on: September 22, 2019

High-Content Screening Assay for the Identification of Antibody-Dependent Cellular Cytotoxicity Modifying Compounds

High-Content Screening Assay for the Identification of Antibody-Dependent Cellular Cytotoxicity Modifying Compounds

Published on: August 18, 2023

Related Experiment Videos

Last Updated: Jun 12, 2026

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

Published on: March 3, 2015

A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput

A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput

Published on: September 22, 2019

High-Content Screening Assay for the Identification of Antibody-Dependent Cellular Cytotoxicity Modifying Compounds

High-Content Screening Assay for the Identification of Antibody-Dependent Cellular Cytotoxicity Modifying Compounds

Published on: August 18, 2023

Area of Science:

Molecular Biology
Cancer Research
Pharmacology

Background:

CCAAT/enhancer-binding protein delta (C/EBPδ) has dual roles in cancer, promoting progression in some cancers but limiting it in pancreatic ductal adenocarcinoma (PDAC).
Targeting C/EBPδ offers clinical potential, but few compounds are known to modulate its activity.

Purpose of the Study:

To identify novel small molecules that modulate C/EBPδ transcriptional activity.
To understand the regulatory mechanisms of C/EBPδ in pancreatic ductal adenocarcinoma (PDAC).

Main Methods:

High-throughput compound screening using a novel eGFP reporter system.
Pathway enrichment analysis to identify targeted pathways.
Validation of identified compounds in PDAC cell models.

Main Results:

Identified 22 potent inducers and 18 inhibitors of C/EBPδ activity from 1402 small molecule inhibitors.
Cell cycle inhibition generally increased C/EBPδ activity, while PI3K/Akt/mTOR inhibitors decreased it.
Four multi-cyclin-dependent kinase (CDK) inhibitors, potent C/EBPδ activators, limited PDAC cell clonal expansion.

Conclusions:

This study provides a valuable set of C/EBPδ-modulating compounds for preclinical research.
Findings enhance understanding of C/EBPδ regulation, particularly in PDAC.
Cell cycle regulation is a key mechanism influencing C/EBPδ activity in PDAC.