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Related Experiment Video

Updated: May 30, 2025

Fluorescence Lifetime Macro Imager for Biomedical Applications
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Multispectral laser-scanning pulse-sampling fluorescence lifetime system for large-scale tissue imaging.

Xuhui Liu, Julien Bec, Xiangnan Zhou

    Optics Letters
    |January 31, 2025
    PubMed
    Summary

    A new multispectral laser-scanning pulse-sampling fluorescence lifetime imaging (LSPS-FLIm) system enables rapid, high-resolution imaging of large tissue samples. This advanced LSPS-FLIm technology offers centimeter-scale fields of view for biological and clinical applications.

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    Area of Science:

    • Biomedical Optics
    • Fluorescence Imaging
    • Microscopy

    Background:

    • Fluorescence lifetime imaging (FLIm) is crucial for analyzing biological tissues.
    • Existing FLIm systems often face limitations in field of view, resolution, or speed.
    • There is a need for advanced imaging systems capable of examining large tissue specimens with high detail.

    Purpose of the Study:

    • To develop and characterize a novel multispectral laser-scanning pulse-sampling fluorescence lifetime imaging (LSPS-FLIm) system.
    • To enable rapid, high-resolution imaging of large tissue specimens.
    • To assess the system's versatility and performance across various biological samples.

    Main Methods:

    • Implementation of a multispectral laser-scanning pulse-sampling fluorescence lifetime imaging system.
    • Characterization of the system's field of view (6 × 15 cm²) and spatial resolution (~17.5 µm).
    • Testing with diverse fluorescent dyes, endogenous tissue fluorophores, and various tissue specimens.

    Main Results:

    • The LSPS-FLIm system achieved centimeter-scale imaging with high spatial resolution.
    • Demonstrated versatility in resolving morphological and molecular features in diverse tissue types.
    • Successfully distinguished complex microstructures within tissue specimens.

    Conclusions:

    • The developed LSPS-FLIm system offers a significant advancement for imaging large tissue specimens.
    • It provides high-quality imaging with rapid acquisition speeds and minimal tissue damage.
    • LSPS-FLIm shows great promise for future biological and clinical applications.