Lignin-based nanoenzyme doped hydrogel for NO-enhanced chemodynamic therapy of bacterial infections

The infections triggered by bacteria often cause wound deterioration, and the development of safe and effective antimicrobial treatment is always highly desired. In this paper, naturally derived lignin was aminated to generate surface group functionalized lignin nanoparticles (NPs), efficiently loading Ag NPs and adsorbing L-arginine, to construct lignin-based nanoenzyme (SALL), which achieves synergistic antimicrobial treatment by chemodynamic therapy and NO gas therapy, while the dose of silver was decreased. The SALL is dispersed in eco-friendly hydrogel constructed using keratin and chitosan through realigned disulfide bond and diverse intermolecular interactions, the prepared SALL@K/C hydrogel has ideal rheological property, and strong adhesion capacity facilitating active bacteria capture, ensuring that the bacteria were immobilized within the effective range of reactive oxygen species and NO. The inhibition rates of S. aureus and E. coli were 98.5 % and 97.8 %, respectively. Meanwhile, the SALL@K/C hydrogel could achieve the delivery of hydrophobic drug curcumin for inhibiting inflammation. The study highlights a well-designed nanoenzyme-loaded hydrogel with excellent antibacterial, hemostatic, and antiinflammatory properties, offering new ideas for nanoenzyme design and antimicrobial hydrogel development.