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Updated: May 29, 2025

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
Published on: January 14, 2016
Properties of the Ureaplasma parvum structural maintenance of chromosomes protein related to its interaction with DNA
Natalia A Rumyantseva1, Vladimir M Shutov1, Dina G Belenkaia1
1Peter the Great St.Petersburg Polytechnic University, Institute of Biomedical Systems and Biotechnology, Polytechnicheskaya 29,Saint Petersburg 195251, Russia.
Abstract:
SMC (Structural Maintenance of Chromosomes) ATPase proteins are integral components of complexes bearing the same name, crucial for the spatial organization of DNA across diverse life forms, spanning bacteria, archaea, and eukaryotes. It is proposed that in bacteria, SMC complexes facilitate DNA compaction through loop extrusion and aid in the segregation of daughter nucleoids. In this paper, the properties of the SMC ATPase protein from Ureaplasma parvum were investigated by using a spectrum of methods, including conventional biochemical methods as well as advanced single-molecule techniques. Our findings reveal distinctive properties of this protein compared to its extensively studied homologue from Bacillus subtilis. Notably, our results suggest that U. parvum Smc ATPase facilitates DNA compaction even in the absence of ATP.
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