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Updated: May 11, 2026
Pharmacologic Induction of Epidermal Melanin and Protection Against Sunburn in a Humanized Mouse Model
Published on: September 7, 2013
Typha pollen extract inhibit melanogenesis via α-MSH/MC1R signaling pathway in B16 and melasma mouse model
Shiqian Zheng1, Rongrong Deng1, Shengjun Xie2
1Guangdong Botanical Beauty Care Biotechnology Co., Ltd, Guangzhou, China.
Abstract:
The aim of this study was to investigate the effects of Typha Pollen extract on melanogenesis in B16 and melasma mouse model. The effects of TP extract on B16 viability, melanin content of B16, MC1R content and TYR activity were studied by MTT assay, NaOH method, ELISA assay and dopa oxidation assay, respectively. In melasma mouse model, the effects of TP extract on MC1R content, MITF content and TYR activity were studied by ELISA assay, immunofluorescence staining and dopa oxidation assay, respectively. The results showed that the appropriate concentration of TP extract does not affect cell viability, and that TP extract inhibit B16 melanin, MC1R content and TYR activity. The results showed that TP extract inhibit skin MC1R content, MITF content and TYR activity. TP extract inhibit B16 melanin, inhibit proteins of α-MSH/MC1R signaling pathway such as MC1R, MITF and TYR. So TP extract is a natural and effective potential whitening agent.
Insights
Typha Pollen extract effectively inhibits melanin production and key proteins in the α-MSH/MC1R signaling pathway. This natural extract shows potential as a safe and effective skin whitening agent.
Area of Science:
- Dermatology
- Cosmetic Science
- Pharmacology
Background:
- Melanogenesis is a complex process regulated by signaling pathways.
- Hyperpigmentation disorders like melasma are a significant concern.
- Natural compounds are increasingly explored for cosmetic applications.
Purpose of the Study:
- To evaluate the effects of Typha Pollen (TP) extract on melanogenesis.
- To assess TP extract's efficacy in B16 melanoma cells and a melasma mouse model.
- To investigate TP extract's impact on key regulatory proteins and enzymes involved in melanin synthesis.
Main Methods:
- Cell viability was assessed using MTT assay.
- Melanin content was quantified using the NaOH method.
- Protein levels (MC1R, MITF) were measured by ELISA and immunofluorescence staining.
- Tyrosinase (TYR) activity was determined via dopa oxidation assay.
Main Results:
- TP extract did not affect B16 cell viability at appropriate concentrations.
- TP extract significantly inhibited melanin production in B16 cells.
- TP extract reduced MC1R content and TYR activity in both B16 cells and the melasma model.
- TP extract decreased MITF content in the melasma mouse model.
Conclusions:
- Typha Pollen extract effectively inhibits melanogenesis by targeting the α-MSH/MC1R signaling pathway.
- TP extract reduces melanin synthesis, MC1R, MITF, and TYR activity.
- TP extract is a promising natural ingredient for skin whitening applications.
