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Best practices for cryopreserving sperm in Nonhuman Primates: a systematic review and meta-analysis
Niloofar Sadeghi1,2, Ndifereke Uboh1, Corinna N Ross2
1Department of Neuroscience, Developmental and Regenerative Biology, The University of Texas at San Antonio, San Antonio, TX, 78249, USA.
Scientific Reports
|January 31, 2025
Summary
Cryopreservation negatively impacts nonhuman primate (NHP) sperm quality, reducing motility and viability. Rapid freezing methods show the greatest recovery of motile NHP sperm, offering insights for optimizing protocols.
Area of Science:
- Veterinary Science
- Reproductive Biology
- Biomedical Research
Background:
- Nonhuman primates (NHPs) are crucial preclinical research models, facing increasing demand.
- Efficient cryopreservation of NHP sperm is vital to expand research availability and alleviate demand.
Purpose of the Study:
- To systematically review and meta-analyze the effects of cryopreservation on NHP sperm parameters.
- To identify optimal cryopreservation strategies for NHP sperm.
Main Methods:
- Systematic literature search of PubMed, Scopus, and Web of Science databases up to June 2024.
- Inclusion of 32 original studies comparing cryopreserved NHP sperm parameters to fresh sperm.
- Meta-analysis to quantify the impact of cryopreservation on sperm motility and viability.
Main Results:
- All 32 studies reported negative effects of cryopreservation on NHP sperm parameters.
- Significant decreases in sperm motility and viability were observed post-thawing.
- Rapid freezing methods yielded the highest recovery of motile NHP sperm.
- Sperm collection method did not significantly impact recovery, but species variability was noted.
Conclusions:
- Cryopreservation significantly impairs NHP sperm quality, particularly motility and viability.
- Rapid freezing techniques are most effective for preserving NHP sperm function.
- Further research is needed to optimize NHP sperm cryopreservation protocols to support biomedical research.
