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Related Experiment Video

Updated: May 16, 2026

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Accelerating isotope dilution LC-MS-based desmosine quantification for estimating elastin turnover.

Elena Kuzmanova1, Angela McIntyre1, Maaz Syed2

  • 1Systems Medicine, School of Medicine, University of Dundee, Dundee, UK.

Bioanalysis
|February 1, 2025
PubMed
Summary
This summary is machine-generated.

A new rapid assay significantly shortens desmosine analysis time to 30 minutes. This breakthrough enables faster assessment of elastin degradation, a key biomarker for mortality risk in aging and disease.

Keywords:
Desmosineaortic aneurysmaortic dissectionmicro solid phase extractionmicrowavestable isotope dilution LC-MS/MS

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biomarker Discovery

Background:

  • Circulating total desmosine indicates systemic elastin degradation, a biomarker for mortality risk in diseases and aging.
  • Current analytical methods for desmosine are time-consuming, exceeding 23 hours, limiting clinical utility.
  • Stable isotope dilution liquid chromatography-mass spectrometry (LC-MS) is a key technique for desmosine quantification.

Purpose of the Study:

  • To develop a significantly faster method for analyzing circulating total desmosine.
  • To reduce the assay turnover time of the stable isotope dilution LC-MS desmosine assay.

Main Methods:

  • Investigated microwave-assisted acid hydrolysis for faster sample preparation.
  • Evaluated direct injection following solid-phase extraction to streamline the workflow.
  • Optimized acid hydrolysis temperature and time, and solid-phase extraction elution volume.

Main Results:

  • Achieved an overall assay time of approximately 30 minutes using microwave-assisted hydrolysis and optimized solid-phase extraction.
  • Validated the assay with good intra-day and inter-day precision and accuracy.
  • Demonstrated significantly higher desmosine concentrations in acute aortic dissection patients compared to controls.

Conclusions:

  • Rapid desmosine analysis is feasible using microwave-assisted hydrolysis and streamlined solid-phase extraction.
  • The developed rapid assay enhances the potential clinical application of desmosine as a mortality risk biomarker.
  • This advancement facilitates quicker insights into elastin degradation in various disease states.