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Related Concept Videos

MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

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Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
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lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
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Related Experiment Video

Updated: May 29, 2025

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy
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Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

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Liposome-Mediated MicroRNA Delivery: An Additional Layer of Gene Network Regulation and Nuclear Reprogramming

Navid Ghasemzadeh1, Fatemeh Pourrajab1, Ali Dehghani Firoozabadi2

  • 1Department of Clinical Biochemistry and Molecular Biology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Iranian Biomedical Journal
|February 1, 2025
PubMed
Summary

Liposomes effectively deliver microRNAs (miRNAs) to induce pluripotency in human mesenchymal stem cells (hMSCs). This novel cell reprogramming method, using liposomal microRNAs (LP-miRs), offers a safer alternative to transduction for regenerative medicine applications.

Keywords:
LiposomesReprogrammingTranscription factorsmicroRNAs

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Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells
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Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells

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Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
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Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

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Related Experiment Videos

Last Updated: May 29, 2025

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy
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Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

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Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells
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Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
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Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

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Area of Science:

  • Biotechnology
  • Stem Cell Biology
  • Molecular Medicine

Background:

  • MicroRNA (miRNA)-mediated cell engineering presents a novel approach for cell reprogramming and generating patient-specific tissues.
  • Optimizing non-transduction reprogramming methods minimizes tumorigenesis risk in reprogrammed cells.
  • This study investigates liposomes for miRNA delivery to regulate gene networks and facilitate nuclear reprogramming.

Purpose of the Study:

  • To explore liposomes as vehicles for delivering miRNAs into cells.
  • To investigate the role of miRNAs in regulating gene networks and nuclear reprogramming.
  • To assess the potential of liposomal microRNA delivery for cell reprogramming.

Main Methods:

  • Utilized cationic liposomal nanoparticles for miRNA delivery into human mesenchymal stem cells (hMSCs).
  • Examined the induction of pluripotency factors (OCT4, SOX2, NANOG) using quantitative polymerase chain reaction (qPCR).

Main Results:

  • miR-302a and miR-34a were found to regulate pluripotency by interacting with OCT4, SOX2, and NANOG.
  • Lipoplexes with miR-302a increased OCT4 expression, while miR-34a decreased it.
  • Pluripotency precursors were successfully induced by delivering liposomal microRNA (LP-miRs).

Conclusions:

  • Liposomal microRNAs (LP-miRs) can influence cell reprogramming and lineage conversion.
  • These findings advance the understanding of pluripotency regulation.
  • LP-miRs hold potential for regenerative medicine applications.