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Updated: May 29, 2025

A Guide to Generating and Using hiPSC Derived NPCs for the Study of Neurological Diseases
Published on: February 21, 2015
Generation of a human induced pluripotent stem cell line (UNIFEi001-A) from a patient with Spinocerebellar ataxia
Mariangela Pappadà1, Sara Melija1, Martina Facchini1
1Department of Environmental and Prevention Sciences, University of Ferrara, 44121 Ferrara, Italy.
Abstract:
Spinocerebellar ataxia type 1 (SCA1) is one of six SCAs related to the expansion of a CAG repeat, encoding glutamine. Induced pluripotent stem cells were generated by reprogramming fibroblasts isolated from a patient affected by SCA1, through the transduction of three viral vectors derived from a non-integrative virus. UNIFEi001-A cell line presents an ESC-like morphology, is vector-free, expresses pluripotency markers, presents a normal karyotype and can differentiate into the three germ layers. These cells can be further differentiated into neural stem cells and neurons to serve as a cellular model.
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