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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
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A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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Updated: May 29, 2025

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
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Functional immunological assays

Attila Kumánovics1, Medhat Askar2, Amir A Sadighi Akha1

  • 1Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, United States.

Journal of Immunological Methods
|February 1, 2025
PubMed
Summary

No abstract available in PubMed .

Keywords:
ApoptosisBasophil activationComplement assaysCytokine measurementHaemophagocytic lymphohistiocytosisLaboratory developed testsLymphocyte proliferationOxidative burstPhosphorylation assays

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