Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

3.0K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.0K
Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

3.1K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.1K
Hematopoiesis01:21

Hematopoiesis

5.1K
The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
5.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modeling the chondrocyte-derived osteoblasts formation process reveals its molecular signature and regulation network.

Bone research·2026
Same author

Cellular quiescence uncouples the proteome from the transcriptome in neural stem cells.

The EMBO journal·2026
Same author

Remodeling of the bone marrow vasculature induced by venetoclax and azacitidine damage.

Blood·2026
Same author

Human TET2-Mutant Clonal Hematopoiesis Expansion Is Driven by Distinct Inflammatory Signaling Responses in Stem Cells Versus Myeloid Progeny.

Blood cancer discovery·2025
Same author

Disruption of NANOG-driven epithelial-mesenchymal transition (EMT) and self-renewal restores drug sensitivity in colorectal cancer.

Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy·2025
Same author

Acute myeloid leukaemia cells express high levels of androgen receptor but do not depend on androgen signaling for survival.

Leukemia·2025

Related Experiment Video

Updated: May 29, 2025

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
12:03

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

Published on: July 8, 2012

18.6K

Methodologic considerations on how to identify human hematopoietic stem cells.

Taylor Hinchly1, Dominique Bonnet2, Fernando Anjos-Afonso3

  • 1Haematopoietic Signalling Group, European Cancer Stem Cell Institute, School of Biosciences, Cardiff University, Cardiff, United Kingdom.

Experimental Hematology
|February 1, 2025
PubMed
Summary
This summary is machine-generated.

This study refines methods for isolating specific human hematopoietic stem cells (HSCs), denoted as CD34+CD38-CD45RA-CD90+/- endothelial protein C receptor (EPCR)+ HSCs. Careful experimental design and antibody selection are crucial for improving HSC purity and reproducibility.

More Related Videos

Isolation Method for Long-Term and Short-Term Hematopoietic Stem Cells
06:41

Isolation Method for Long-Term and Short-Term Hematopoietic Stem Cells

Published on: May 19, 2023

1.6K
Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells
10:20

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Published on: March 24, 2023

1.3K

Related Experiment Videos

Last Updated: May 29, 2025

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
12:03

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

Published on: July 8, 2012

18.6K
Isolation Method for Long-Term and Short-Term Hematopoietic Stem Cells
06:41

Isolation Method for Long-Term and Short-Term Hematopoietic Stem Cells

Published on: May 19, 2023

1.6K
Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells
10:20

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Published on: March 24, 2023

1.3K

Area of Science:

  • Hematology
  • Stem Cell Biology
  • Immunophenotyping

Background:

  • Human hematopoietic stem cells (HSCs) are critical for blood formation and transplantation.
  • Previous isolation methods yielded mixed populations, necessitating improved purity for research and clinical applications.
  • A specific HSC subset, CD34+CD38-CD45RA-CD90+/- endothelial protein C receptor (EPCR)+ HSCs, has been identified.

Purpose of the Study:

  • To evaluate and optimize the methodology for isolating highly pure human CD34+ HSCs.
  • To explore the impact of different antibody clones, conjugates, cell sources, and additional surface markers on HSC purity.
  • To provide guidance on experimental planning and reagent selection to minimize pitfalls in HSC isolation.

Main Methods:

  • Comparative analysis of various antibody clones and conjugates for cell surface marker detection.
  • Evaluation of different cell sources for HSC isolation.
  • Inclusion of additional cell surface antigens (integrin-α6, CLEC9A, GPRC5C) in the isolation panels.
  • Assessment of purity of isolated HSC populations using flow cytometry.

Main Results:

  • Differences in antibody clones and conjugates significantly affect the purity of isolated EPCR+ HSCs.
  • The inclusion of integrin-α6, CLEC9A, and GPRC5C markers can further refine HSC enrichment.
  • Specific reagent pitfalls were identified, impacting reproducibility.
  • Optimized protocols demonstrated enhanced purity of the target HSC population.

Conclusions:

  • Methodology refinement, including careful antibody selection and experimental design, is essential for reproducible isolation of pure human CD34+ EPCR+ HSCs.
  • Understanding reagent limitations and potential pitfalls is critical for successful HSC enrichment.
  • This work provides a foundation for improved standardization and further advancements in HSC isolation techniques.