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Related Concept Videos

Improving Translational Accuracy02:07

Improving Translational Accuracy

Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
Improving Translational Accuracy02:07

Improving Translational Accuracy

Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...

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Quantitative imaging outperforms No-reflow in predicting functional outcomes in a translational stroke model.

Justine Debatisse1, Lucie Chalet2, Omer Faruk Eker3

  • 1Université Claude Bernard Lyon1, CarMeN Laboratory, INSERM, INRAE, Bât. B13, Groupement Hospitalier Est, 59 Boulevard Pinel, Lyon, France.

Neurotherapeutics : the Journal of the American Society for Experimental Neurotherapeutics
|February 1, 2025
PubMed
Summary

Quantitative PET-MRI imaging reveals early microvascular dysfunction after stroke treatment. This imaging signature can predict poor outcomes and infarct growth, aiding in identifying neuroprotection targets.

Keywords:
Acute ischemic strokeBlood-brain barrierNo-reflowOxygen metabolismPET-MRIThrombectomy

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Area of Science:

  • Neuroscience
  • Radiology
  • Cardiovascular System

Background:

  • Microvascular dysfunction and no-reflow are key factors in secondary brain damage after acute ischemic stroke (AIS).
  • Endovascular mechanical thrombectomy (EVT) is a primary treatment for AIS, but outcomes are affected by microvascular integrity.
  • Quantitative imaging is crucial for characterizing early microvascular changes post-revascularization.

Purpose of the Study:

  • To utilize quantitative PET-MRI imaging to characterize early microvascular damage in a non-human primate model of AIS undergoing EVT.
  • To correlate imaging findings with infarct growth and neurological outcomes one week post-EVT.
  • To identify early imaging biomarkers predictive of poor outcomes and potential neuroprotection targets.

Main Methods:

  • A preclinical non-human primate model of AIS underwent EVT.
  • Quantitative PET-MRI imaging was performed during occlusion (perfusion, diffusion) and post-revascularization (perfusion, diffusion, blood-brain barrier permeability, oxygen metabolism).
  • Neurological scores and lesion growth were assessed one week post-EVT to define poor outcomes.

Main Results:

  • Early post-EVT hypoperfusion was detected by PET in 67% and by MRI Tmax in 90% of cases within the acute lesion.
  • Thirty-eight percent of cases experienced a poor outcome, all of whom exhibited no-reflow phenomena on imaging.
  • Diffusion ADC alterations and elevated oxygen extraction fraction (OEF) correlated with PET no-reflow; prolonged Tmax and high BBB Ktrans independently predicted poor outcomes.

Conclusions:

  • Quantitative PET-MRI provides an early imaging signature of microvascular dysfunction post-EVT in AIS.
  • This signature, including hypoperfusion, no-reflow, and BBB permeability, effectively predicts infarct growth and poor neurological outcomes.
  • The findings highlight the potential of early quantitative imaging to guide treatment and identify targets for neuroprotection.