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Updated: Jun 23, 2026

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

Introduce a novel, extremely sensitive aptamer against staphylococcal enterotoxin type D.

Arezoo Fallah¹, Abbas Ali Imani Fooladi², Hamid Sedighian²

¹Department of Bacteriology and Virology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

International Journal of Biological Macromolecules

|February 2, 2025

Summary

Researchers developed a novel DNA aptamer for detecting staphylococcal enterotoxin D (SED), a key cause of food poisoning. This aptamer offers a sensitive and specific tool for identifying this dangerous foodborne pathogen.

Keywords:

ELASA SELEX SPR Staphylococcal enterotoxin D Staphylococcus aureus ssDNA-aptamer

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Area of Science:

Biotechnology
Molecular Biology
Food Safety

Background:

Staphylococcus aureus is a major foodborne pathogen causing Staphylococcal food poisoning (SFP) through enterotoxins.
Rapid detection of staphylococcal enterotoxins (SEs) is crucial for public health.
Aptamers offer a promising alternative to antibodies for diagnostics due to their stability and cost-effectiveness.

Purpose of the Study:

To isolate high-affinity single-stranded DNA (ssDNA) aptamers specifically targeting staphylococcal enterotoxin D (SED).

Main Methods:

Systematic evolution of ligands by exponential enrichment (SELEX) was employed to identify aptamers.
Enzyme-linked apta-sorbent assay (ELASA) and surface plasmon resonance (SPR) were used for validation and affinity assessment.

Main Results:

SELEX yielded aptamers with significant binding affinity for SED.
Aptamer 1 demonstrated high specificity for SED with a dissociation constant (K_D) of 4.4 ± 2.26 nM.
The limit of detection (LOD) for SED using this aptamer was 45 nM.

Conclusions:

The developed aptamer, when used in an ELASA system, provides enhanced specificity, sensitivity, and reproducibility for SED detection.
This novel aptamer holds potential for advanced diagnostic platforms targeting S. aureus enterotoxins.