Comparing the cannabidiol-induced transcriptomic profiles in human and mouse Sertoli cells

Area of Science:

• Reproductive Toxicology
• Cell Biology
• Molecular Biology

Background:

• Cannabidiol (CBD), a Cannabis sativa L. cannabinoid, is used for epilepsy treatment.
• Concerns exist regarding CBD's male reproductive toxicity, observed in animal models.
• Previous studies showed CBD inhibits proliferation in human and mouse Sertoli cells.

Purpose of the Study:

• To investigate molecular changes induced by CBD in mouse Sertoli TM4 cells.
• To compare CBD's effects on mouse Sertoli TM4 cells with primary human Sertoli cells.
• To identify species-specific responses of Sertoli cells to CBD.

Main Methods:

• RNA-sequencing analysis of mouse Sertoli TM4 cells treated with CBD.
• Transcriptomic data analysis to identify molecular pathways affected by CBD.
• Comparative analysis of transcriptomic profiles between mouse and human Sertoli cells.

Main Results:

• CBD induced apoptosis in mouse Sertoli TM4 cells, unlike cellular senescence in human Sertoli cells.
• Transcriptomic analysis revealed immune and cellular stress responses in mouse Sertoli TM4 cells.
• Major differences in molecular changes were observed between mouse and human Sertoli cells following CBD exposure.

Conclusions:

• Mouse Sertoli TM4 cells and primary human Sertoli cells exhibit distinct molecular responses to CBD.
• CBD-induced male reproductive toxicity may involve different mechanisms in different species.
• Further research is needed to elucidate the precise mechanisms of CBD's reproductive toxicity.