Emerging non-invasive MRI techniques for glymphatic system assessment in neurodegenerative disease

The discovery of the glymphatic system has revolutionized our understanding of brain physiology, particularly in waste clearance and fluid dynamics within the central nervous system. This pathway, essential for nutrient distribution and waste removal, operates predominantly during sleep and has been implicated in neurodegenerative diseases like Alzheimer's and Parkinson's. Recent advances in non-invasive MRI techniques, including diffusion tensor imaging along the perivascular space (DTI-ALPS), perivascular space (PVS) analysis, and free water (FW) indices, have improved our ability to study glymphatic function and its alterations in disease states. This review discusses the glymphatic system's ultrastructure, physiology, and the latest imaging methods to assess this critical pathway. We highlight how these non-invasive MRI techniques can enhance the understanding of glymphatic function in health and disease, with a focus on neurodegenerative conditions. By integrating insights from current research, this review underscores the diagnostic and therapeutic implications of glymphatic dysfunction. Understanding these mechanisms can pave the way for novel strategies to enhance waste clearance and improve neurological health, offering potential benefits for early diagnosis and intervention in neurodegenerative diseases.