Effect of Feeding Environmental Enrichment on Lesser Anteaters' Behavior, Space Use and Food Selectivity

Studying behavioral response to management and housing conditions provides specific evidence to enhance animal welfare. Feeding environmental enrichment (FEE) is a powerful strategy to meet welfare challenges. Lesser anteaters' (Tamandua tetradactyla; n = 7) response to FEE was assessed by analyzing behavior and space use through focal sampling every 5 minutes/24 hours using cameras. Study 1 (S1): space use of enclosure zones (shelter = SZ, climbing structures = CSZ and ground = GZ) was characterized without FEE during 5 days using the modified Spread of Participation index (SPI). Study 2 (S2) used an ABA experimental design during 18 days (A=pre- and post-enrichment and B = enrichment). The FEE was applied in CSZ, the least utilized zone revealed in S1. S1: The lesser anteaters utilized the zones differently (modified SPI = 0.74 ± 0.02), and stayed mostly in the GZ. S2: An increment in feeding during FEE was detected, but it did not modify total activity, most natural behavior, repetitive locomotion or space use. The electivity index indicated that the routine diet was over-utilized and enrichment items were under-utilized. The provision of enrichment items created new foraging opportunities, improving the behavioral domain of animal welfare.