Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>In Situ</i> Construct of Zn-In Alloy Layer by In(BF<sub>4</sub>)<sub>3</sub> Additive to Stabilize Zn Anode and Realize Ultra-High Reversible Zinc-Air Batteries.

Journal of the American Chemical Society·2026
Same author

In Situ Synchronized SERS-SEIRAS Unveils Cation-Regulated Interfacial Water and Intermediates in the Oxygen Reduction Reaction.

Angewandte Chemie (International ed. in English)·2026
Same author

Spatial imaging of water oxidation on single-particle catalysts.

Nature nanotechnology·2026
Same author

Radical-Mediated Dynamic Reconstruction of Ni-N-C Single-Atom Catalysts for Wide-Potential CO<sub>2</sub>-to-CO Electroreduction.

Journal of the American Chemical Society·2026
Same author

Topological Data Analysis in Materials Science: Principles, Machine Learning Integration, and Application Landscapes.

Chemical reviews·2026
Same author

Subsurface hydrogen as a hidden driver of copper surface reconstruction in CO<sub>2</sub> electroreduction.

National science review·2026

Related Experiment Video

Updated: May 29, 2025

Author Spotlight: Advancements and Applications in Nanoparticle Synthesis Through Laser Ablation in Liquids
06:15

Author Spotlight: Advancements and Applications in Nanoparticle Synthesis Through Laser Ablation in Liquids

Published on: June 16, 2023

1.8K

A high sensitivity prostate-specific antigen SERS detection platform based on laser resonance nanoparticles.

Shi-Ying Fu1, Shanshan Xu1, Hongmei Li1

  • 1The Key Laboratory of Urinary Tract Tumours and Calculi, Department of Urology, The First Affiliated Hospital of Xiamen University, School of Medicine, College of Chemistry and Chemical Engineering, College of Energy, and Discipline of Intelligent Instrument and Equipment, Xiamen University, Xiamen 361005, China. taowang@xmu.edu.cn.

Nanoscale
|February 3, 2025
PubMed
Summary

This study introduces a rapid, low-cost SERS immunoassay for prostate-specific antigen (PSA) detection in serum. The method offers a 10-minute turnaround time, making it ideal for early prostate cancer screening in underserved areas.

More Related Videos

Surface-enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detecting Microscopic Ovarian Cancer via Folate Receptor Targeting
07:54

Surface-enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detecting Microscopic Ovarian Cancer via Folate Receptor Targeting

Published on: March 25, 2019

8.1K
Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

20.2K

Related Experiment Videos

Last Updated: May 29, 2025

Author Spotlight: Advancements and Applications in Nanoparticle Synthesis Through Laser Ablation in Liquids
06:15

Author Spotlight: Advancements and Applications in Nanoparticle Synthesis Through Laser Ablation in Liquids

Published on: June 16, 2023

1.8K
Surface-enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detecting Microscopic Ovarian Cancer via Folate Receptor Targeting
07:54

Surface-enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detecting Microscopic Ovarian Cancer via Folate Receptor Targeting

Published on: March 25, 2019

8.1K
Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

20.2K

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Oncology

Background:

  • Accurate quantification of cancer biomarkers in serum is crucial for early cancer diagnosis and treatment.
  • Prostate-specific antigen (PSA) is a key biomarker for prostate cancer diagnosis and prognosis, with elevated levels indicating potential malignancy.
  • Current hospital methods for PSA detection are time-consuming, expensive, and have limited accessibility in rural healthcare settings.

Purpose of the Study:

  • To develop and validate a novel, rapid, and cost-effective immunoassay for PSA detection in clinical serum using surface-enhanced Raman scattering (SERS).
  • To assess the clinical applicability and performance of the SERS-based method compared to established hospital diagnostic techniques.
  • To provide a portable and accessible diagnostic tool suitable for widespread use, including in township hospitals.

Main Methods:

  • An immunoassay was designed utilizing magnetic beads and SERS nanotags for PSA detection.
  • The SERS method was evaluated using 75 male clinical serum samples, with a focus on the clinically relevant range of 4.0-10.0 ng mL-1.
  • Results were compared against those obtained from the hospital's electrochemical luminescence (ECL) system.

Main Results:

  • The SERS-based immunoassay achieved a detection limit of 0.029 ng mL-1 for patient serum samples.
  • The developed method demonstrated good agreement with the conventional ECL system used in clinical laboratories.
  • The SERS assay provides results within 10 minutes, significantly reducing diagnostic waiting times.

Conclusions:

  • The SERS-based immunoassay is a highly sensitive, rapid, and portable method for detecting PSA in clinical serum.
  • This technology offers a cost-effective alternative to current hospital-based diagnostics, enhancing accessibility for prostate cancer screening.
  • The method shows significant potential for market adoption in rural and township healthcare facilities in China and beyond.