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Mitochondria

Mitochondria

Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...

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Updated: May 4, 2026

Methods to Assess Subcellular Compartments of Muscle in C. elegans

Methods to Assess Subcellular Compartments of Muscle in C. elegans

Published on: November 13, 2014

Imaging and Quantifying Mitochondrial Morphology in C. elegans During Aging.

Juri Kim¹, Maxim Averbukh¹, Athena Alcala¹

¹Leonard Davis School of Gerontology, University of Southern California.

Journal of Visualized Experiments : Jove

|February 3, 2025

Summary

This summary is machine-generated.

Mitochondrial function declines with age, altering cell structure. This study introduces reliable GFP imaging and quantification methods in C. elegans to study these aging-related mitochondrial changes.

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Last Updated: May 4, 2026

Methods to Assess Subcellular Compartments of Muscle in C. elegans

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Imaging and Quantifying Mitochondrial Morphology in C. elegans During Aging

Imaging and Quantifying Mitochondrial Morphology in C. elegans During Aging

Published on: January 17, 2025

Area of Science:

Cell Biology
Aging Research
Genetics

Background:

Mitochondria are vital organelles for cellular energy production and regulation of metabolism, proliferation, and signaling.
Mitochondrial dysfunction is linked to aging and stress, manifesting as morphological changes.
The nematode C. elegans is a valuable model for studying aging due to its transparency and short lifespan.

Purpose of the Study:

To present a robust and reliable method for imaging mitochondrial morphology in C. elegans.
To minimize errors and variability in mitochondrial imaging during aging studies.
To recommend a method for quantifying age-related mitochondrial morphological changes.

Main Methods:

Development and application of single-copy, matrix-localized GFP constructs for mitochondrial imaging in C. elegans.
Focus on experimentally controllable factors to reduce variability in imaging.
Utilizing the mitoMAPR tool for quantitative analysis of mitochondrial morphology.

Main Results:

Single-copy, matrix-localized GFP constructs provide a robust method for visualizing mitochondrial morphology.
Controlled experimental factors significantly reduce variability in mitochondrial imaging across replicates and studies.
The mitoMAPR method effectively quantifies age-associated mitochondrial morphological alterations.

Conclusions:

Single-copy GFP constructs offer a reliable approach for studying mitochondrial morphology in C. elegans.
Standardizing experimental conditions is crucial for accurate aging studies.
mitoMAPR is a valuable tool for analyzing mitochondrial dynamics during the aging process.