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Updated: May 29, 2025
In Vivo Imaging of Reactive Oxygen Species in a Murine Wound Model
Published on: November 17, 2018
Proanthocyanidin capsules remodel the ROS microenvironment via regulating MAPK signaling for accelerating diabetic
Shaoyin Wei1,2,3, Dong Yang2, Zeyu Shou4
1School of Ophthalmology and Optometry, Eye Hospital, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, 325035, China.
Abstract:
Defective diabetic wound healing is a major clinical challenge, where hyperglycemia at the wound site induces excessive reactive oxygen species (ROS) which activate the MAPK pathway (particularly p38 MAPK), resulting in sustained release of inflammatory factors and cellular damage/apoptosis. Polyphenols are efficient ROS scavengers which reduce the level of inflammation at the wound site and promote wound healing, but the low bioavailability limits their biomedical application. This study developed a simple and highly efficient method for preparing proanthocyanidin (PC) capsules through hydrogen bonding and hydrophobic interactions among PC molecules. PC capsules can continuously scavenge free radicals and release proanthocyanidins, significantly enhancing their bioavailability. A single dose of PC capsules accelerates wound healing in diabetic mice by regulating the p38 MAPK signaling cascade, reducing inflammatory mediator concentration, inhibiting cell apoptosis, and remodeling the wound microenvironment. This research makes an important contribution to the field of enhancing polyphenol bioavailability for wound healing and reveals the potential of modulating the MAPK pathway for treating other inflammation and oxidative stress-related diseases.
Insights
This study developed proanthocyanidin (PC) capsules to improve bioavailability for enhanced diabetic wound healing. The capsules effectively scavenge free radicals and regulate inflammatory pathways, accelerating recovery in diabetic mice.
Area of Science:
- Biomedical Engineering
- Materials Science
- Pharmacology
Background:
- Defective diabetic wound healing is a significant clinical issue.
- Hyperglycemia in diabetic wounds increases reactive oxygen species (ROS), activating the MAPK pathway and causing inflammation and cell damage.
- Polyphenols show promise for wound healing but suffer from poor bioavailability.
Purpose of the Study:
- To develop a method for preparing proanthocyanidin (PC) capsules with enhanced bioavailability.
- To investigate the efficacy of PC capsules in accelerating diabetic wound healing.
- To explore the underlying mechanisms of PC capsule action on the MAPK pathway and wound microenvironment.
Main Methods:
- Developed PC capsules using hydrogen bonding and hydrophobic interactions.
- Administered a single dose of PC capsules to diabetic mice with wounds.
- Assessed wound healing progression, inflammatory mediator levels, cell apoptosis, and MAPK pathway activation (p38 MAPK).
Main Results:
- PC capsules demonstrated continuous free radical scavenging and sustained proanthocyanidin release, significantly improving bioavailability.
- A single dose of PC capsules accelerated wound healing in diabetic mice.
- Treatment regulated the p38 MAPK signaling cascade, reduced inflammatory mediators, inhibited apoptosis, and remodeled the wound microenvironment.
Conclusions:
- PC capsules offer a promising strategy to enhance polyphenol bioavailability for treating diabetic wound healing.
- Modulating the MAPK pathway via PC capsules presents a potential therapeutic approach for inflammation and oxidative stress-related diseases.
- This study contributes to developing novel treatments for complex wound healing challenges.
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