Related Experiment Video

Updated: May 29, 2025

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization
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High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

Published on: June 28, 2012

11.6K

Three annotated chromosome-level de novo genome assemblies of Lomentospora prolificans provide evidence for a

Nina T Grossman, Yunfan Fan, Aleksey V Zimin

    Biorxiv : the Preprint Server for Biology
    |February 3, 2025
    PubMed

    Insights

    Lomentospora prolificans, a drug-resistant fungus causing severe infections, has had its genome fully sequenced. This breakthrough offers hope for developing new antifungal treatments and understanding its evolution.

    Area of Science:

    • Mycology
    • Genomics
    • Infectious Diseases

    Background:

    • Lomentospora prolificans is a fungal pathogen causing severe, often fatal, infections in immunocompromised individuals.
    • Existing antifungal drugs are largely ineffective against L. prolificans due to inherent resistance.

    Purpose of the Study:

    • To generate chromosome-level de novo genome assemblies for Lomentospora prolificans.
    • To provide the first complete genome assembly of L. prolificans.
    • To aid in the discovery of novel antifungal drugs and understand fungal evolution.

    Main Methods:

    • Chromosome-level de novo genome assembly of three L. prolificans strains.
    • Genome annotation.
    • Pulsed-field gel electrophoresis for assembly confirmation.

    Main Results:

    • The first complete genome assembly of L. prolificans was achieved.
    • The genome comprises 11 nuclear and 1 mitochondrial chromosome, totaling 36.7-37.1 Mb.
    • A chromosomal translocation was identified in one strain, supported by contig variations.

    Conclusions:

    • The availability of complete L. prolificans genomes is crucial for antifungal drug development.
    • These genomic resources provide insights into the evolutionary history of this challenging pathogen.

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