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Updated: May 29, 2025

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Feature-Driven Whole-Tissue Imaging with Subcellular Resolution.

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    We developed Multiscale Cleared Tissue Axially Swept Light-Sheet Microscopy (MCT-ASLM) for imaging intact tissues across scales. This advanced microscopy platform bridges subcellular details to whole tissue structures, aiding biological discovery.

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    Area of Science:

    • Biological imaging
    • Microscopy techniques
    • Tissue biology

    Background:

    • Understanding the link between subcellular events and tissue-wide phenomena is crucial.
    • Existing imaging methods face limitations in scale and resolution for complex biological systems.

    Purpose of the Study:

    • To introduce Multiscale Cleared Tissue Axially Swept Light-Sheet Microscopy (MCT-ASLM).
    • To enable imaging of intact tissues from centimeter-scale down to submicron resolution.
    • To facilitate the study of hierarchical organization and complex biological processes.

    Main Methods:

    • Developed MCT-ASLM, combining large field-of-view imaging with high-resolution capabilities.
    • Utilized axially swept light-sheet microscopy for cleared tissue samples.
    • Enabled both human-guided and autonomous imaging modes.

    Main Results:

    • MCT-ASLM captures fields of view up to 21 mm at micron-scale resolution.
    • The platform can transition to submicron (~300 nm) isotropic resolution for targeted imaging.
    • Demonstrated applications in mapping neuronal circuits, visualizing kidney innervation, and examining tumor microenvironments.

    Conclusions:

    • MCT-ASLM provides a versatile tool for bridging subcellular and tissue-level scales.
    • This technology advances the study of hierarchical organization in biological samples.
    • MCT-ASLM offers a powerful method for investigating how local biological events influence global phenomena.