Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Intracellular Signaling Affects Focal Adhesions

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...

Cell Adhesion Molecules - Types and Functions

Cell Adhesion Molecules - Types and Functions

Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily involved...

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...

Laminins are the Adhesive Proteins of Basal Lamina

Laminins are the Adhesive Proteins of Basal Lamina

Laminins are heterotrimeric proteins with high molecular mass found in the extracellular matrix. Each laminin molecule is composed of three chains, viz. alpha, beta, and gamma, coded by five, four, and three paralogous genes, respectively. Laminins are categories based on the compositions of the three chains.
In humans, the five forms of alpha chains are LAMA 1, LAMA 2, LAMA 3, LAMA 4, and LAMA 5. The four forms of beta chains are LAMB 1, LAMB 2, LAMB 3, and LAMB 4. The three forms of gamma...

Cadherins in Tissue Organization

Cadherins in Tissue Organization

The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...

Anchoring Junctions

Anchoring Junctions

Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Protease suppression by native I9 inhibitor improves recombinant protein production in Trichoderma reesei.

Bioresource technology·2026

Same author

Functional interplay between heat shock protein 90 (HSP90) and heat shock factors (HSFs).

Cell stress & chaperones·2026

Same author

Customizable FDM-based zebrafish larva mold for live imaging.

Biology open·2026

Same author

Fast label-free live imaging with FlowVision reveals key principles of cancer cell arrest on endothelial monolayers.

The EMBO journal·2026

Same author

PhotoFiTT: a quantitative framework for assessing phototoxicity in live-cell microscopy experiments.

Nature communications·2025

Same author

Regulation of cell dynamics by rapid integrin transport through the biosynthetic pathway.

The Journal of cell biology·2025

Search research articles

Related Experiment Video

Updated: May 29, 2025

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Published on: June 13, 2014

Nuclear talin-1 provides a bridge between cell adhesion and gene expression.

Alejandro J Da Silva^1,2, Hendrik S E Hästbacka^1,2, Mikael C Puustinen^1,2

¹Faculty of Science and Engineering, Cell Biology, Åbo Akademi University, 20520 Turku, Finland.

|February 3, 2025

Summary

Talin-1 (TLN1) is known to activate integrins, but it also localizes to the nucleus and associates with chromatin. Nuclear TLN1 influences gene expression and cell-cell adhesion in human breast cells.

Keywords:

cell biology cellular physiology molecular biology molecular interaction properties of biomolecules

More Related Videos

Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner

Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner

Published on: December 10, 2015

Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies

Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies

Published on: March 19, 2014

Related Experiment Videos

Last Updated: May 29, 2025

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Published on: June 13, 2014

Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner

Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner

Published on: December 10, 2015

Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies

Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies

Published on: March 19, 2014

Area of Science:

Cell Biology
Molecular Biology
Genomics

Background:

Talin-1 (TLN1) is primarily recognized for its role in integrin activation and mechanotransduction at focal adhesions.
Its subcellular localization is not exclusively confined to the cell periphery.

Purpose of the Study:

To investigate the nuclear localization and function of Talin-1.
To explore the impact of nuclear Talin-1 on gene expression and cellular behavior.

Main Methods:

Subcellular fractionation and confocal microscopy were used to determine TLN1 localization.
Small interfering RNA (siRNA) was employed for gene silencing studies.
Expression of a nuclear-localized TLN1 fusion protein was utilized to assess functional impact.

Main Results:

Talin-1 (TLN1) was found to localize within the nucleus and associate with chromatin in human cell lines.
Depletion of endogenous TLN1 by siRNA resulted in significant alterations in gene expression profiles.
Nuclear accumulation of TLN1 modified the expression of specific genes and disrupted cell-cell cluster formation.

Conclusions:

Talin-1 (TLN1) possesses a nuclear function beyond its established role at focal adhesions.
Nuclear TLN1 plays a role in regulating gene expression and influencing cell-cell adhesion dynamics.