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Nuclear talin-1 provides a bridge between cell adhesion and gene expression.
Alejandro J Da Silva1,2, Hendrik S E Hästbacka1,2, Mikael C Puustinen1,2
1Faculty of Science and Engineering, Cell Biology, Åbo Akademi University, 20520 Turku, Finland.
Iscience
|February 3, 2025
Summary
Talin-1 (TLN1) is known to activate integrins, but it also localizes to the nucleus and associates with chromatin. Nuclear TLN1 influences gene expression and cell-cell adhesion in human breast cells.
Area of Science:
- Cell Biology
- Molecular Biology
- Genomics
Background:
- Talin-1 (TLN1) is primarily recognized for its role in integrin activation and mechanotransduction at focal adhesions.
- Its subcellular localization is not exclusively confined to the cell periphery.
Purpose of the Study:
- To investigate the nuclear localization and function of Talin-1.
- To explore the impact of nuclear Talin-1 on gene expression and cellular behavior.
Main Methods:
- Subcellular fractionation and confocal microscopy were used to determine TLN1 localization.
- Small interfering RNA (siRNA) was employed for gene silencing studies.
- Expression of a nuclear-localized TLN1 fusion protein was utilized to assess functional impact.
Main Results:
- Talin-1 (TLN1) was found to localize within the nucleus and associate with chromatin in human cell lines.
- Depletion of endogenous TLN1 by siRNA resulted in significant alterations in gene expression profiles.
- Nuclear accumulation of TLN1 modified the expression of specific genes and disrupted cell-cell cluster formation.
Conclusions:
- Talin-1 (TLN1) possesses a nuclear function beyond its established role at focal adhesions.
- Nuclear TLN1 plays a role in regulating gene expression and influencing cell-cell adhesion dynamics.
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