Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Localized regulation of cell junction mRNAs is required for epithelial cell integrity.

RNA (New York, N.Y.)·2026
Same author

Systematic surveillance of SARS-CoV-2 reveals dynamics of variant mutagenesis and transmission in a large urban population.

Nature communications·2024
Same author

Canada's contributions to RNA research: past, present, and future perspectives.

Biochemistry and cell biology = Biochimie et biologie cellulaire·2024
Same author

Integrated multi-omics analysis of zinc-finger proteins uncovers roles in RNA regulation.

Molecular cell·2024
Same author

ZO-1 interacts with YB-1 in endothelial cells to regulate stress granule formation during angiogenesis.

Nature communications·2024
Same author

CircRNAome of Childhood Acute Lymphoblastic Leukemia: Deciphering Subtype-Specific Expression Profiles and Involvement in <i>TCF3::PBX1</i> ALL.

International journal of molecular sciences·2024
Same journal

Isolation of Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Stepwise Optimization of a Matrigel-Based In Vitro Angiogenesis Assay for Reproducible and Quantifiable 2D-Tube Formation Using HUVECs.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Quantifying Mechanical Properties of Fresh Ovarian Tissue with Optical Brillouin Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

3D Chromatin Architecture During Early Development: New Methods and New Findings.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Metabolic Plasticity in Embryogenesis Throughout the Lens of NAD<sup></sup>.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: May 29, 2025

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

22.7K

Exploring Extracellular Vesicle Transcriptomic Diversity Through Long-Read Nanopore Sequencing.

Juan-Carlos A Padilla1,2, Seda Barutcu1, Gabrielle Deschamps-Francoeur1

  • 1Institut de Recherches Cliniques de Montréal (IRCM), Montréal, QC, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|February 3, 2025
PubMed
Summary
This summary is machine-generated.

Nanopore long-read sequencing enables analysis of full-length RNA within extracellular vesicles (EVs), offering new insights into intercellular communication. This method overcomes limitations of short-read sequencing for comprehensive EV RNA profiling.

Keywords:
EVsExtracellular vesiclesLong-read sequencingNanopore sequencingOxford Nanopore TechnologiesPoly-APolyadenylated transcriptomeRNA sequencingTranscript isoforms

More Related Videos

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

13.6K
Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
05:45

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Published on: March 11, 2020

8.7K

Related Experiment Videos

Last Updated: May 29, 2025

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

22.7K
Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

13.6K
Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
05:45

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Published on: March 11, 2020

8.7K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genomics

Background:

  • Extracellular vesicles (EVs) mediate intercellular communication via RNA cargo.
  • Short-read sequencing fragments RNA, limiting analysis of EV transcriptomes.
  • The presence and integrity of full-length RNAs in EVs are not well understood.

Purpose of the Study:

  • To present a protocol for analyzing full-length RNA in EVs using nanopore sequencing.
  • To overcome limitations of traditional RNA sequencing methods for EV research.
  • To explore the integrity and isoform diversity of EV RNA cargo.

Main Methods:

  • Extracellular vesicle (EV) RNA purification.
  • Complementary DNA (cDNA) library preparation for nanopore sequencing.
  • Oxford Nanopore Technologies (ONT) MinION sequencing platform utilization.

Main Results:

  • Nanopore long-read sequencing enables analysis of full-length EV RNA.
  • This approach provides deeper insights into EV RNA integrity and isoform diversity.
  • A comprehensive protocol for EV RNA sequencing using ONT is detailed.

Conclusions:

  • Long-read nanopore sequencing is a powerful tool for EV RNA research.
  • This method enhances understanding of EV-mediated intercellular communication.
  • The presented protocol facilitates detailed analysis of EV transcriptomes.