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Related Concept Videos

Applications Of NMR In Biology01:25

Applications Of NMR In Biology

3.7K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
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Nuclear Magnetic Resonance (NMR): Overview01:07

Nuclear Magnetic Resonance (NMR): Overview

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Nuclear magnetic resonance (NMR) is a phenomenon exhibited by certain nuclei that can absorb characteristic radio frequency radiation under certain conditions. NMR has been extensively applied in molecular spectroscopy and medical diagnostic imaging. In both these applications, the molecule or subject under study is placed in a magnetic field and irradiated with radio frequency energy.
NMR spectroscopy generates a spectrum where the characteristic absorption frequencies of the sample are...
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Nuclear Overhauser Enhancement (NOE)01:07

Nuclear Overhauser Enhancement (NOE)

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Irradiation of a spin-active nucleus causes an increase or decrease in the signal intensity of neighboring nuclei that are not necessarily chemically bonded or involved in J-coupling.  This phenomenon, called the Nuclear Overhauser Enhancement (NOE), results from through-space interactions between the nuclear spins. The NOE effect decreases with increasing internuclear distance and is generally not observed beyond 4 angstroms. In NOE, dipole-dipole interactions between neighboring...
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Atomic Nuclei: Magnetic Resonance01:05

Atomic Nuclei: Magnetic Resonance

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The number of nuclear spins aligned in the lower energy state is slightly greater than those in the higher energy state. In the presence of an external magnetic field, as the spins precess at the Larmor frequency, the excess population results in a net magnetization oriented along the z axis. When a pulse or a short burst of radio waves at the Larmor frequency is applied along the x axis, the coupling of frequencies causes resonance and flips the nuclear spins of the excess population from the...
605
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

158
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
158
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

652
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
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Related Experiment Video

Updated: May 29, 2025

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
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Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

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Optical widefield nuclear magnetic resonance microscopy.

Karl D Briegel1,2, Nick R von Grafenstein1,2, Julia C Draeger1,2

  • 1Technical University of Munich, TUM School of Natural Sciences, Department of Chemistry, Lichtenbergstraße 4, 85748, Garching bei München, Germany.

Nature Communications
|February 3, 2025
PubMed
Summary

This study introduces a novel optical widefield NMR microscopy technique using diamond quantum sensors. It enables high-resolution imaging of nuclear magnetic resonance signals across a wide field of view, advancing microscopy capabilities.

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Area of Science:

  • Quantum Sensing
  • Microscopy
  • Spectroscopy

Background:

  • Traditional magnetic resonance imaging (MRI) faces limitations in real-time, wide-field imaging.
  • Capturing nuclear magnetic resonance (NMR) signals directly on a camera has been a significant challenge in microscopy.

Purpose of the Study:

  • To develop a novel optical widefield NMR microscopy method.
  • To overcome the limitations of conventional NMR imaging by utilizing quantum sensing technology.

Main Methods:

  • Employing nitrogen-vacancy (NV) centers in diamond as quantum sensors to convert NMR signals into optical signals.
  • Utilizing a high-speed camera to capture the optically converted NMR signals.
  • Demonstrating imaging in microfluidic structures.

Main Results:

  • Achieved ~10 μm resolution across a ~235 × 150 μm² area using optical widefield NMR microscopy.
  • Each camera pixel recorded a full NMR spectrum, providing rich data on amplitude, phase, magnetic fields, and gradients.
  • Successfully imaged NMR signals in microfluidic devices.

Conclusions:

  • The developed optical widefield NMR microscopy offers a powerful new tool for multifaceted imaging in physical and life sciences.
  • This technique fuses optical microscopy and NMR, enabling detailed, wide-field analysis of minute structures and processes.
  • Opens new avenues for advanced imaging applications previously inaccessible with traditional methods.