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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

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Related Experiment Video

Updated: May 29, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Nature Inspired Delivery Vehicles for CRISPR-Based Genome Editing.

Elizabeth Maria Clarissa^1,2, Mamata Karmacharya¹, Hyunmin Choi^1,2

¹Center for Algorithmic and Robotized Synthesis, Institute for Basic Science (IBS), UNIST-gil 50, Ulsan, 44919, Republic of Korea.

Small (Weinheim an Der Bergstrasse, Germany)

|February 4, 2025

Summary

This summary is machine-generated.

Efficient and safe delivery systems are crucial for Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) genome editing therapies. Nanocarriers, including viral vectors and extracellular vesicles, enhance CRISPR

Keywords:

CRISPR delivery genome editing membrane vesicles precision therapy

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Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles "Nanoblades"

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Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles "Nanoblades"

Published on: March 31, 2021

Area of Science:

Biotechnology and Genetic Engineering
Nanomedicine
Molecular Biology

Background:

CRISPR genome editing offers revolutionary therapeutic potential for genetic diseases.
Effective delivery of CRISPR components is a major challenge for clinical translation.
Nanocarrier systems are emerging as key solutions for efficient and safe CRISPR delivery.

Purpose of the Study:

To review the integration of CRISPR genome editors with various nanocarrier systems.
To highlight recent advancements in nanocarrier-mediated CRISPR delivery.
To discuss future directions for therapeutic genome editing.

Main Methods:

Exploration of natural and synthetic nanocarrier-mediated delivery systems.
Analysis of viral vectors, extracellular vesicles (EVs), engineered cellular membrane particles, liposomes, and nanoparticles.
Focus on carrier efficiency, specificity, and immunogenicity.

Main Results:

Nanocarriers enhance CRISPR efficacy through improved efficiency, specificity, and reduced immunogenicity.
Synthetic carriers offer reproducibility and customizable functions for CRISPR delivery.
Viral vectors provide high transduction efficiency, while EVs offer immune evasion and targeted delivery.

Conclusions:

The combination of CRISPR with nanocarriers represents a significant advancement in therapeutic genome editing.
Various nanocarrier strategies show promise for overcoming delivery challenges in CRISPR-based therapies.
Further research into nanocarrier systems will drive the clinical application of genome editing.