Related Experiment Video

Updated: May 29, 2025

Serum and Plasma Copy Number Detection Using Real-time PCR
09:21

Serum and Plasma Copy Number Detection Using Real-time PCR

Published on: December 15, 2017

11.3K

Influence of DNA Copy Number Aberrations in ABC Transporter Family Genes on the Survival of Patients with Primary

Matvey Mikhailovich Tsyganov1,2, Marina Konstantinovna Ibragimova1,2,3, Evgeny Olegovich Rodionov1

  • 1Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences; 5, Kooperativny Street, Tomsk, Russian Federation.

Current Cancer Drug Targets
|February 4, 2025
PubMed
Abstract

Insights

Chromosomal aberrations and gene expression of ABC transporters impact survival in non-small cell lung cancer (NSCLC). Specific gene alterations, like ABCB1 deletion, correlate with significantly improved metastatic-free survival (MFS).

Area of Science:

  • Oncology
  • Genetics
  • Molecular Biology

Background:

  • Previous studies linked ABC transporters gene expression to therapy efficacy.
  • Mechanisms beyond gene expression, such as chromosomal aberrations, are less explored in non-small cell lung cancer (NSCLC).

Purpose of the Study:

  • To investigate chromosomal aberrations in all 49 ABC transporters genes in NSCLC.
  • To assess the expression levels of selected ABC genes.
  • To determine the correlation between these genetic alterations and patient survival.

Main Methods:

  • Microarray analysis of DNA and RNA from 104 NSCLC patient samples.
  • Assessment of gene expression and chromosomal aberrations (deletions, amplifications) of ABC genes.
  • Correlation analysis with metastatic-free survival (MFS) after surgery and chemotherapy.

Main Results:

  • Hypoexpression of ABCC1, ABCC2, and ABCG1 was linked to significantly higher MFS (p=0.01).
  • ABCB1 deletion correlated with 100% MFS (p=0.001), while ABCG1 amplification showed survival rates below 45% (p<0.0001).
  • ABCA11 deletion was associated with low MFS (38%), contrasting with ABCB9 amplification (55% survival). ABCC3 and ABCC10 amplification also showed reduced survival.

Conclusions:

  • Both gene expression and chromosomal aberrations of ABC transporters are associated with patient survival in NSCLC.
  • These genetic alterations may serve as potential biomarkers for predicting metastatic-free survival.
Keywords:
ABC transportersDNA copy number aberrationsNon-small cell lung canceradjuvant chemotherapygene expressionmetastasis-free survival

More Related Videos

Author Spotlight: Advancements in Molecular Biomarker Testing for Non-Squamous Non-Small Cell Lung Cancer07:59

Author Spotlight: Advancements in Molecular Biomarker Testing for Non-Squamous Non-Small Cell Lung Cancer

Published on: September 8, 2023

1.0K
Detection of Copy Number Alterations Using Single Cell Sequencing09:45

Detection of Copy Number Alterations Using Single Cell Sequencing

Published on: February 17, 2017

11.6K

Related Concept Videos

Combination Therapies and Personalized Medicine02:50

Combination Therapies and Personalized Medicine

Combining two or more treatment methods increases the life span of cancer patients while reducing damage to vital organs or tissue from the overuse of a single treatment. Combination therapy also targets different cancer-inducing pathways, thus reducing the chances of developing resistance to treatment.
The combination of the drug acetazolamide and sulforaphane is a good example of combination therapy to treat cancer. The cells in the interior of a large tumor often die due to the hypoxic and...
4.8K
Comparing Copy Number Variations and SNPs02:26

Comparing Copy Number Variations and SNPs

Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
17.1K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
7.4K
Treatment Resistant Cancers02:56

Treatment Resistant Cancers

Cancer is the second leading cause of death in the United States. A cancer cell is genetically unstable and hence can mutate faster. They can also modify their microenvironment and escape immune surveillance. The difficulties in treating cancer are further compounded by the emergence of rapid resistance to anticancer drugs. The most common ways to attain resistance in cancer cells include alteration in drug transport and metabolism, modification of drug target, elevated DNA damage response, or...
3.2K