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Dual-Template Imprinted Polyaniline Designed by Response Surface Methodology
V D Gorlo1, P S Pidenko1, N A Burmistrova1
1Institute of Chemistry, Saratov State University, Saratov, Russia.
Journal of Separation Science
|February 4, 2025
Summary
This study developed a novel dual-template molecularly imprinted polyaniline for simultaneously detecting quercetin and horseradish peroxidase. Response surface methodology optimized synthesis for high selectivity in solid-phase extraction.
Area of Science:
- Analytical Chemistry
- Materials Science
- Polymer Chemistry
Background:
- Molecularly imprinted polymers (MIPs) offer selective recognition capabilities.
- Simultaneous imprinting of both low and high molecular weight compounds presents a significant challenge.
- Polyaniline (PANI) is a conductive polymer with potential for sensor applications.
Purpose of the Study:
- To develop and optimize a novel dual-template molecularly imprinted polyaniline (MIP) for simultaneous selective recognition of quercetin and horseradish peroxidase.
- To investigate the role of horseradish peroxidase in aniline polymerization for MIP synthesis.
- To establish a methodology for creating MIPs selective to both low and high molecular weight analytes concurrently.
Main Methods:
- Response surface methodology (RSM) was employed to optimize MIP synthesis conditions.
- Molecularly imprinted polyaniline layers were fabricated on microtitration plates.
- Solid-phase extraction (SPE) was utilized to evaluate the selectivity and capacity of the MIPs.
- Imprinting factors (IFs) were calculated to quantify selectivity.
Main Results:
- RSM successfully predicted optimal synthesis conditions, yielding high selectivity for quercetin (IF=2.4).
- The optimized MIP-modified microtitration plate demonstrated high selectivity for both quercetin (IF=2.3) and horseradish peroxidase (IF=24.6) in model solutions.
- Achieved sorption capacities were 0.7 mg g⁻¹ for quercetin and 1.2 mg g⁻¹ for horseradish peroxidase.
- Experimental results closely matched RSM predictions.
Conclusions:
- A novel methodology for synthesizing dual-template MIPs selective to both low and high molecular weight compounds was successfully established.
- The developed polyaniline-based MIPs show significant potential for simultaneous solid-phase extraction of quercetin and horseradish peroxidase.
- RSM is an effective tool for optimizing MIP synthesis conditions to achieve high specificity and performance.
