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Radiological Investigation III: Pulmonary Angiogram and PET Scan01:13

Radiological Investigation III: Pulmonary Angiogram and PET Scan

Radiological investigations are paramount in the diagnosis and management of various pulmonary diseases. Two essential investigations are the Pulmonary Angiogram and the Positron Emission Tomography (PET) Scan.
Pulmonary Angiogram
A Pulmonary Angiogram is an invasive procedure involving injecting a contrast medium through a catheter threaded into the pulmonary artery or the right side of the heart to visualize the pulmonary vasculature. Computed Tomography (CT) scans have mainly replaced this...

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Related Experiment Video

Updated: Jul 8, 2026

Intra-cardiac Side-Firing Light Catheter for Monitoring Cellular Metabolism using Transmural Absorbance Spectroscopy of Perfused Mammalian Hearts
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A Closed Circulation Langendorff Heart Perfusion Method for Cardiac Drug Screening.

C Deflers1, R Donate Puertas, P Lechene

  • 1Université Paris-Saclay, INSERM, Signaling and Cardiovascular Pathophysiology, Orsay, France. guillaume.pidoux@inserm.fr.

Physiological Research
|February 4, 2025
PubMed
Summary

A novel constant-flow recirculating circuit system for ex vivo heart perfusion offers a cost-effective method for assessing drug cardiotoxicity. This method provides stable cardiac parameters and reliable screening for therapeutic agents.

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Area of Science:

  • Cardiovascular Pharmacology
  • Drug Discovery and Development
  • Toxicology

Background:

  • Cardiovascular diseases cause significant global morbidity and mortality, posing an economic burden.
  • Inadequate cardiovascular safety and efficacy of drugs lead to market withdrawal or late-stage development failures.
  • There is a critical need for cost-effective assays to screen for acute drug cardiotoxicity early in development.

Purpose of the Study:

  • To introduce and validate a constant-flow with a recirculating circuit (CFCC) system for ex vivo heart perfusion.
  • To demonstrate the stability of cardiac parameters and the system's efficiency in drug screening.
  • To compare the CFCC system with traditional open perfusion methods.

Main Methods:

  • Utilized the Langendorff isolated heart perfusion technique with a novel constant-flow recirculating circuit (CFCC).
  • Assessed cardiac hemodynamic parameters, including contractile function and heart rate, under basal and stimulated conditions.
  • Validated the system by screening therapeutic agents, including isoproterenol stimulation and propranolol blockade.

Main Results:

  • Cardiac parameters remained stable over time in the CFCC mode.
  • The recycled ex vivo perfusion system yielded comparable results to the traditional open system under basal and isoproterenol-stimulated conditions.
  • The CFCC system successfully demonstrated proof of concept for therapeutic agent screening, with propranolol effectively countering isoproterenol's effects.

Conclusions:

  • The constant-flow recirculating circuit (CFCC) system is a stable, cost-effective, and efficient alternative for ex vivo heart perfusion.
  • This method reduces perfusate volume, enabling broader compound screening for cardiovascular safety.
  • The CFCC system is validated for early-stage drug discovery and cardiotoxicity screening.