Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vibration Error Compensation of LiDAR Imaging with the Aiding of INS for Precise Navigation.

Sensors (Basel, Switzerland)·2026
Same author

Inhalable ROS-Responsive Nanospray Activates PPAR-γ to Restore Macrophage Mitochondrial Homeostasis and Attenuate Radiation-Induced Lung Injury.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

A Synergistic C<sub>2+</sub> Alcohols/Olefins-Intermediated Pathway Boosts CO<sub>2</sub> Hydrogenation to Aromatics.

Angewandte Chemie (International ed. in English)·2026
Same author

Exotic Critical States as Fractional Fermi Seas in the One-Dimensional Bose Gas.

Physical review letters·2026
Same author

NaCl-zwitterion enhanced chitosan-based hydrogels with super compressibility, conductivity, and stretchability as wearable strain and pressure sensors.

International journal of biological macromolecules·2026
Same author

Mitochondria-targeting organoselenium theranostic radioprotectors for simultaneous treatment and imaging of radiation-induced liver injury.

Theranostics·2026

Related Experiment Video

Updated: May 29, 2025

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
12:24

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Published on: July 17, 2012

12.3K

A peptide-based fluorescent bioprobe for EphA2-overexpressing tumor targeting and image-guided surgical resection.

Xudong Yu1, Jianfei Jin2, Yun Si2

  • 1Department of Gastroenterology, Yiwu Central Hospital, the Affiliated Yiwu Hospital of Wenzhou Medical University, Yiwu, Zhejiang Province, China.

Bioorganic & Medicinal Chemistry
|February 4, 2025
PubMed
Summary

A novel near-infrared fluorescent bioprobe, YK80, targets Ephrin receptor A2 (EphA2) for enhanced visualization in fluorescence-guided surgery (FGS). This targeted approach improves accuracy in detecting colorectal tumors, outperforming non-targeted agents like indocyanine green (ICG).

Keywords:
EphA2-overexpressing tumorFluorescence-guided surgeryFluorescent probespeptide-based

More Related Videos

MR Molecular Imaging of Prostate Cancer with a Small Molecular CLT1 Peptide Targeted Contrast Agent
06:54

MR Molecular Imaging of Prostate Cancer with a Small Molecular CLT1 Peptide Targeted Contrast Agent

Published on: September 3, 2013

11.2K
A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
05:51

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

Published on: March 17, 2023

1.7K

Related Experiment Videos

Last Updated: May 29, 2025

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
12:24

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Published on: July 17, 2012

12.3K
MR Molecular Imaging of Prostate Cancer with a Small Molecular CLT1 Peptide Targeted Contrast Agent
06:54

MR Molecular Imaging of Prostate Cancer with a Small Molecular CLT1 Peptide Targeted Contrast Agent

Published on: September 3, 2013

11.2K
A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
05:51

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

Published on: March 17, 2023

1.7K

Area of Science:

  • Biomedical Engineering
  • Medical Imaging
  • Translational Medicine

Background:

  • Fluorescence-guided surgery (FGS) enhances surgical precision by providing real-time visualization of lesions and tumor margins.
  • Current non-targeted contrast agents lack specificity, limiting their effectiveness in complex surgical scenarios.
  • Targeted fluorescent probes are needed to improve the accuracy and efficacy of FGS.

Purpose of the Study:

  • To develop and characterize a novel near-infrared fluorescent bioprobe, YK80, for targeted imaging in FGS.
  • To evaluate the targeting ability and efficacy of YK80 in preclinical models of colorectal cancer.
  • To compare the performance of YK80 with a non-targeted agent, indocyanine green (ICG).

Main Methods:

  • Design and synthesis of YK80, a bioprobe utilizing a heptamethine cyanine dye and a peptide targeting Ephrin receptor A2 (EphA2).
  • Characterization of YK80's optical properties, pharmacokinetics, and binding affinity to EphA2.
  • In vivo evaluation of YK80's targeting ability in mouse models with colorectal tumors, compared to ICG.

Main Results:

  • YK80 demonstrated high affinity (KD ≈ 100 nM) for EphA2-expressing cancer cells.
  • YK80 exhibited excellent targeting capability in mouse models of colorectal cancer.
  • Indocyanine green (ICG) showed no specific targeting capability towards cancer cells or tumors.

Conclusions:

  • YK80 shows significant potential as a targeted contrast agent for image-guided surgery.
  • The development of YK80 represents a successful example for advancing medical imaging and surgical techniques.
  • This targeted approach could significantly improve surgical outcomes and reduce cancer recurrence.