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Murine chapparvovirus (MuCPV)/mouse kidney parvovirus (MKPV) eradication by cross foster rederivation
Ibrahim Faseeh1,2, Vatthana Vongphakham3, Patrick Sharp1,3,4
1Animal Resources Centre, Murdoch, Western Australia, Australia.
Abstract:
When diagnostic tests became available for the detection of mouse kidney parvovirus (MKPV), also known as murine chapparvovirus (MuCPV), we undertook a facility-wide screening to determine the prevalence of this novel agent in the Animal Resources Centre. MKPV was present only in the Customs Strains, specific-pathogen-free (SPF) barrier area, which was the only animal holding area that received mice imports directly from non-approved vendors. Based on this information, the knowledge and understanding of the viral epizootiology, and the testing methods available, we decided to eradicate MKPV after consultation with the researchers impacted. To eradicate MKPV from the barrier, we took a multi-modal approach composed of testing and separating positive and negative cages; and cross-foster (XF) rederivation. The test and separate approach was unsuccessful due to the animal housing and husbandry set up in the SPF barrier area given that MKPV is an environmentally stable and highly infectious agent. However, with an XF rederivation method, we were able to successfully rederive 11 out of 16 litters of various genetically modified lines that remained MKPV negative over a 20-week testing period. Our trial indicates that XF rederivation techniques, coupled with strict disinfection, can be used for a growing list of viral and other infectious agents that are highly infectious and persistent in the environment.
Insights
Mouse kidney parvovirus (MKPV) was eradicated from a specific-pathogen-free (SPF) barrier using cross-foster (XF) rederivation. This method, combined with disinfection, offers a solution for controlling persistent viral agents in research settings.
Area of Science:
- Veterinary Virology
- Laboratory Animal Science
- Infectious Disease Control
Background:
- Mouse kidney parvovirus (MKPV), also known as murine chapparvovirus (MuCPV), is a novel viral agent.
- MKPV was detected in the specific-pathogen-free (SPF) barrier area of an animal research facility, primarily in imported mice from non-approved vendors.
Purpose of the Study:
- To determine the prevalence of MKPV in a research facility.
- To eradicate MKPV from the SPF barrier area.
- To evaluate the efficacy of different eradication methods.
Main Methods:
- Facility-wide screening for MKPV using diagnostic tests.
- Implementation of a multi-modal eradication approach including testing, cage separation, and cross-foster (XF) rederivation.
- Monitoring of rederived mice for MKPV negativity over a 20-week period.
Main Results:
- MKPV was exclusively found in the SPF barrier area, linked to imported mice.
- A test-and-separate strategy proved ineffective due to MKPV's environmental stability and high infectivity.
- Cross-foster (XF) rederivation successfully produced 11 MKPV-negative litters from 16 attempts.
Conclusions:
- Cross-foster (XF) rederivation is an effective method for eradicating MKPV from research animal colonies.
- XF rederivation, alongside stringent disinfection, can be applied to control other persistent and highly infectious viral agents.
- This study highlights the importance of import controls and effective rederivation techniques in maintaining pathogen-free research animal populations.
